Tissue dispersion, cell harvest and fluid suspension culture by the use of bacterial neutral protease.
Tissue dispersion, cell harvest and fluid suspension culture by the use of bacterial neutral protease.
复制标题
使用细菌中性蛋白酶进行组织分散、细胞收获和液体悬浮培养。
DOI：
--
复制
发表时间：
1975
期刊：
影响因子：
--
通讯作者：
K. Nitta
中科院分区：
文献类型：
--
作者：
T. Matsumura;T. Yamanaka;S. Hashizume;Y. Irie;K. Nitta
多粘芽孢杆菌的中性蛋白酶可以分散哺乳动物的组织和细胞。在磷酸盐缓冲液、平衡盐溶液或添加血清的组织培养液中，以每毫升200至2000个库尼茨单位的该酶处理后，从几个组织获得原代细胞培养。单层培养物在其早期传代水平或已建立的菌株中枯萎，用该酶处理后收获，并在去除后再次在单层中增殖。在单分散悬浮液中培养的L-929菌株在该酶存在下保持生长。与胰酶不同，该酶在有血清存在的情况下被发现是活性的，在孵育过程中稳定，对细胞几乎没有损伤。
Bacterial neutral protease of Bacillus polymyxa was found to disperse mammalian tissues and cells. Primary cell cultures were obtained from several tissues after treatments with 200 to 2,000 Kunitz unit per ml of this protease in either a phosphate buffer solution, a balanced salt solution or a tissue culture medium supplemented with serum. Monolayer cultures wither in their early passage levels or of established strains were harvested by a treatment with this protease, and proliferated again in monolayer after its removal. A growing culture of strain L-929 was kept in monodisperse suspension in the presence of this protease. In contrast to trypsin, this protease was found active in the presence of serum, stable during incubation and scarcely injured cells.