Determinants for urinary and plasma isoflavones in humans after soy intake
Determinants for urinary and plasma isoflavones in humans after soy intake
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DOI：
10.1207/s15327914nc5002_3
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发表时间：
2004-01-01
影响因子：
2.9
通讯作者：
Hundahl, SA
中科院分区：
文献类型：
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作者：
Franke, AA;Custer, LJ;Hundahl, SA
食用大豆食品导致血液和尿液中的异黄酮（IFL）出现双相模式，在摄入后1-2 h和4-8 h出现峰值，但其原因尚不清楚。在摄入大豆食品、IFL糖苷或糖苷配基后，重复测量血浆和/或尿液中的IFL，口服抗生素（AB）治疗或联合机械肠道准备（AB+MBP）治疗导致肠道植物群轻度或根本减少。当不含（对照）或含AB处理的蛋白质饮料或当IFL糖苷或糖苷配基被消耗时，在血液和/或尿液中观察到典型的双相IFL模式。大豆摄入与AB+MBP或葛根素的消费相结合，导致第二个高峰的移动到更晚的时间。葛根素摄入后第一个峰消失。与对照组相比，AB+MBP后前24小时总尿IFL恢复率降低50%以上，但总体上高出61%。当比较相应时间间隔的曲线下面积时，个体或总尿IFL排泄率与个体或总血浆IFL水平高度相关（r = 0.85-0.91; P < 0.001）。在相同的尿排泄率的三倍以上的染料木素比大豆黄酮留在循环。我们的结论是尿IFL排泄率反映循环IFL水平，大豆黄酮出现在血液中少，尿中比染料木素。第一个和第二个IFL峰分别是小肠和大肠的摄取。在通常的膳食IFL剂量（0.15- 1.5 μ mol/kg体重）下，大肠是主要的摄取部位（90%）。减少肠道植物群延迟IFL的吸收，但总体上导致增加尿液的恢复，因为在肠道中的细菌降解较少。
Consumption of soy foods leads to a biphasic appearance pattern of isoflavones (IFLs) in blood and urine, with peaks appearing at 1-2 h and 4-8 h after intake, but its causes are not understood. IFLs were measured repeatedly from plasma and/or urine after intake of soy foods, IFL glucosides, or aglycons without or with a mildly or radically reduced gut flora as a result of oral antibiotic (AB) treatment, or this combined with mechanical bowel preparation (AB+MBP). The typical biphasic IFL pattern in blood and/or urine was observed when a so), protein drink without (control) or with AB treatment or when IFL glucosides or aglycons were consumed. Soy intake combined with AB+MBP or consumption of puerarin led to a shift of the second peak to much later times. The first peak was absent after puerarin intake. Total urinary IFL recovery was more than 50% lower in the first 24 h, but overall 61% higher after AB+MBP vs. the control. When the area under the curves for corresponding time intervals were compared, individual or total urinary IFL excretion rates were highly correlated with individual or total plasma IFL levels (r = 0.85-0.91; P < 0.001). At the same urinary excretion rate three times more genistein than daidzein remained in the circulation. We conclude that urinary IFL excretion rates reflect circulating IFL levels, with daidzein appearing less in blood and more in urine than genistein. The first and second IFL peaks are due to uptake in the small and large intestine, respectively The latter is the major locus of uptake (90%) at usual dietary IFL doses (0.15-1.5,mumol/kg body weight). A reduced gut flora delayed IFL uptake but led overall to increased urinary recovery because of less bacterial degradation in the intestine.