Sympathetic denervation of one white fat depot changes norepinephrine content and turnover in intact white and brown fat depots.
Sympathetic denervation of one white fat depot changes norepinephrine content and turnover in intact white and brown fat depots.
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DOI：
10.1038/oby.2012.95
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发表时间：
2012-07
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Harris RB
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作者：
Harris RB
交感神经系统调节脂肪细胞代谢已被证实，最近有报道称来自白色脂肪的感觉传入神经在解剖学上与来自白色脂肪的交感传入神经重叠。本文描述的研究描述了完整脂肪垫对雄性NIH瑞士小鼠腹股沟（ING）或附睾（EPI）脂肪选择性交感神经切除术（局部6-羟多巴胺注射）的反应，并为个体白色和棕色脂肪库之间的交流提供了体内证据。对侧ING垫、两个EPI垫、肾周垫和肠系膜垫在去神经后4周显著增大，但当两个ING垫都去神经时，只有囊内棕色脂肪（IBAT）增加。一个或两个EPI垫去神经对脂肪储备重量没有影响。在另一项实验中，在两个EPI或两个ING垫去神经支配两天后，ING、腹膜后、肠系膜和IBAT中的NE转换被抑制。各肥库NE含量降至对照值的10-30%。在去神经支配4周后，尽管完整脂肪垫的NE含量仍在减少，但NE周转量的早期变化与脂肪垫重量之间没有关系。这些数据表明，可能是通过感觉传入纤维和交感传出纤维活动的中枢整合，在单个脂肪垫之间存在通信，从而以统一的方式改变对白色脂肪组织的交感驱动。在特定情况下，对一个神经垫的交感神经传出的移除会引起其他完整神经库的代偿性扩大。
It is well established that the sympathetic nervous system regulates adipocyte metabolism and recently it has been reported that sensory afferents from white fat overlap anatomically with sympathetic efferents to white fat. The studies described here characterize the response of intact fat pads to selective sympathectomy (local 6-hydroxydopamine injections) of inguinal (ING) or epididymal (EPI) fat in male NIH Swiss mice and provide in vivo evidence for communication between individual white and brown fat depots. The contralateral ING pad, both EPI pads, perirenal and mesenteric pads were significantly enlarged four weeks after denervating one ING pad, but only intrascapular brown fat (IBAT) increased when both ING pads were denervated. Denervation of one or both EPI pad had no effect on fat depot weights. In an additional experiment, NE turnover was inhibited in ING, retroperitoneal, mesenteric and IBAT two days after denervation of both EPI or of both ING pads. NE content was reduced to 10-30% of control values in all fat depots. There was no relation between early changes in NE turnover and fat pad weight 4 weeks after denervation, even though the reduction in NE content of intact fat pads was maintained. These data demonstrate that there is communication among individual fat pads, presumably through central integration of activity of sensory afferent and sympathetic efferent fibers,that changes sympathetic drive to white adipose tissue in a unified manner. In specific situations, removal of sympathetic efferents to one pad induces a compensatory enlargement of other intact depots.