A survey of expressed genes in Japanese flounder (Paralichthys olivaceus) liver and spleen.
A survey of expressed genes in Japanese flounder (Paralichthys olivaceus) liver and spleen.
复制标题
日本牙鲆（Paralichthys olivaceus）肝脏和脾脏中表达基因的调查。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
T. Aoki
中科院分区：
文献类型：
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作者：
S. Inoue;B. Nam;I. Hirono;T. Aoki
表达序列标签（ESTs）从日本比目鱼（Paralichthys olivaceus）。我们目前的493个ESTs的单程测序结果从350个克隆的肝脏cDNA和57个ESTs从41个克隆的脾脏cDNA。将cDNA克隆的序列与GenBank数据库中的序列进行比较。202个克隆（51.7%）似乎是完全未知的，很可能代表新描述的基因，而189个克隆（48.3%）是根据数据库中的序列匹配确定的。从肝脏和脾脏cDNA文库中分离出三个未鉴定的序列。然而，肝和脾克隆之间没有相同的序列。
Expressed sequence tags (ESTs) were obtained from Japanese flounder (Paralichthys olivaceus). We present the results of single-pass sequencing of 493 ESTs from 350 clones from liver cDNA and 57 ESTs from 41 clones from spleen cDNA. Sequences of the cDNA clones were compared with sequences in the GenBank database. Two hundred and two clones (51.7%) appeared to be completely unknown and are likely to represent newly described genes, whereas 189 clones (48.3%) were identified based on matches to sequences in the databases. Three of the unidentified sequences were isolated from both the liver and spleen cDNA libraries. However, there were no identical sequences between liver and spleen clones.