ELECTROPHYSIOLOGICAL RESPONSE PATTERNS OF 16 OLFACTORY NEURONS FROM THE TRICHOID SENSILLA TO ODORANT FROM FECAL VOLATILES IN THE LOCUST, Locusta migratoria manilensis
ELECTROPHYSIOLOGICAL RESPONSE PATTERNS OF 16 OLFACTORY NEURONS FROM THE TRICHOID SENSILLA TO ODORANT FROM FECAL VOLATILES IN THE LOCUST, Locusta migratoria manilensis
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DOI：
10.1002/arch.20420
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发表时间：
2011-06-01
影响因子：
2.2
通讯作者：
Zhang, Long
中科院分区：
文献类型：
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作者：
Cui, Xiaojie;Wu, Caihong;Zhang, Long
在世界大部分地区，蝗虫是最严重的农作物害虫。它们主要通过嗅觉线索定位寄主植物，利用触角化学感受器，其中包含嗅觉受体神经元（orn）。尽管这些物种具有巨大的经济价值，但它们的嗅觉神经元在功能水平上的研究很少。本研究采用单感器记录（SSRs）方法，研究了东方飞蝗触角毛状感器对不同化学物质的反应模式。根据毛状感受器的自发峰值振幅，毛状感受器可分为两种类型，分别包含两个或三个orn。这两种结构类型可进一步划分为7个功能亚型。在SSRs期间，蝗虫粪便中存在的九种不同气味被用作刺激物。特别是，苯甲醛在大多数测试的orn中引起抑制反应。此外，当苯甲醛与反式-2-己烯醛或2-庚酮混合时，这些刺激物的兴奋反应被抑制。通过SSRs鉴定了这些orn对9种化学物质的至少16种反应模式，表明蝗虫化学接受的细胞机制具有高度复杂性。(C) 2011 Wiley期刊公司
Locusts are the most serious pests of crops in greater part of the world. They locate their host plants primarily through olfactory cues, using antennal chemosensilla, which house olfactory receptor neurons (ORNs). Despite the great economical interest of these species, their olfactory neurons have been poorly investigated at the functional level. In this study, we have used single sensillum recordings (SSRs) to obtain response patterns of ORNs from the antennal trichoid sensilla to various chemicals in the oriental locust Locusta migratoria. On the basis of their spontaneous spike amplitudes, trichoid sensilla could be distinguished into two types, housing two or three ORNs, respectively. These two structural types could be further classified into seven functional subtypes. Nine different odorants that are present in the locust feces were used as stimulants during SSRs. In particular, benzaldehyde elicited inhibitory responses in most of the ORNs tested. Moreover, in a majority of these ORNs, the excitatory responses obtained with trans-2-hexenal or 2-heptanone was inhibited when benzaldehyde was mixed with these stimulants. At least 16 response patterns of these ORNs to nine chemicals were identified by SSRs, suggesting a high complexity of the cellular mechanisms underlying chemoreception in locusts. (C) 2011 Wiley Periodicals, Inc.