CMOS chip as luminescent sensor for biochemical reactions
CMOS chip as luminescent sensor for biochemical reactions
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DOI：
10.1109/jsen.2003.814653
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发表时间：
2003-06-01
影响因子：
4.3
通讯作者：
Lin, CT
中科院分区：
文献类型：
--
作者：
Lu, U;Hu, BCP;Lin, CT
我们描述了一种新的生化传感方法及其潜在的新的生物传感应用。研制了一种用于生化反应测量的光敏互补金属氧化物半导体芯片，该芯片采用0.5um标准工艺制作。设计了一种以辣根过氧化物酶(HRP)为催化剂的发光酶反应作为测定氢气浓度的平台反应。用标准半导体参数分析仪(HP4145)对过氧化氢(H_2O_2)进行测定。在生化实验室测定了酶反应动力学，并与标准的精密荧光仪(日立F-4500)进行了比较。两种仪器获得了相似的结果。以葡萄糖氧化酶为例，我们进一步证明了HRP平台可以用来测定其他产生H_2O_2的反应。这一结果表明了该芯片在生物测量和各种健康因素的诊断中的重要应用。
We describe a novel biochemical sensing method and its potential new biosensing applications. A light-sensitive complementary metal oxide semiconductor (CMOS) chip prepared through a standard 0.5-mum CMOS process was developed for measuring biochemical reactions. A light producing enzymatic reaction catalyzed by horseradish peroxidase (HRP) was designed as a platform reaction to determine the concentration of hydrogen. peroxide (H2O2) by the CMOS chip with a standard semiconductor parameter analyzer (HP4145). The kinetics of enzymatic reaction were determined and compared with a standard and sophisticated fluorometer (Hitachi F-4500) in a biochemical laboratory. Similar results were obtained by both instruments. Using glucose oxidase as an example, we further demonstrated that the HRP platform can be used to determine other H2O2 producing reactions with the CMOS system. The result points to an important application of the CMOS chip in biological measurements and in diagnosis of various health factors.