The interference of T cell activation by calcium channel blocking agents.
The interference of T cell activation by calcium channel blocking agents.
复制标题
钙通道阻断剂对 T 细胞活化的干扰。
作者：
D. Birx;M. Berger;T. Fleisher
钙离子作为跨膜信使在淋巴细胞活化信号中起重要作用。为了进一步研究该模型，我们评估了钙通道阻滞剂（维拉帕米、硝苯地平和地尔硫卓）对淋巴细胞活化的影响。在这些研究中，我们发现药物以剂量依赖的方式抑制T细胞的增殖和有丝分裂原刺激后某些活化抗原的出现。这似乎是由于加入这些药物后，促分裂原诱导的45 Ca（45 Ca +2）内流明显减少所致。此外，由于IL 2与其受体结合而导致的T细胞增殖也被钙通道阻断剂抑制。这些数据表明，被动钙通道在配体-受体相互作用后T细胞的初始活化和IL 2与其受体结合提供的持续增殖信号中起着关键作用。
Calcium has been identified as having an important role as a transmembrane messenger in the activation signal for lymphocytes. To additionally examine this model, we evaluated the effect of calcium channel blocking drugs (verapamil, nifedipine, and diltiazem) on lymphocyte activation. In these studies we found that the drugs inhibit, in a dose-dependent fashion, the proliferation of T cells and the appearance of certain activation antigens after mitogen stimulation. This appears to result from the marked decrease in mitogen-induced 45calcium (45Ca+2) influx secondary to the addition of these agents. In addition, T cell proliferation resulting from IL 2 binding to its receptor is also suppressed by the calcium channel blocking drugs. These data suggest that the passive calcium channel plays a pivotal role in both the initial activation of T cells after ligand-receptor interaction and the ongoing signal for proliferation provided by IL 2 binding to its receptor.