ULTRASTRUCTURAL-LOCALIZATION OF VIRAL-ANTIGEN IN NUCLEAR INCLUSIONS OF CYTOMEGALOVIRUS INFECTED GUINEA-PIG CELLS
ULTRASTRUCTURAL-LOCALIZATION OF VIRAL-ANTIGEN IN NUCLEAR INCLUSIONS OF CYTOMEGALOVIRUS INFECTED GUINEA-PIG CELLS
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DOI：
10.1007/bf01314706
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
BRIGATI, D
中科院分区：
文献类型：
--
作者：
FONG, CKY;BRIGATI, D
Intranuclear localization of viral antigens in guinea pig cytomegalovirus (GPCMV) infected guinea pig embryo (GPE) cells was investigated by cross-reactive indirect immunoperoxidase and immunoferritin techniques utilizing guinea pig antisera to GPCMC. Following primary fixation with 4% paraformaldehyde, a brief treatment of infected cells with 0.25% trypsin was found to enhance penetration of antibodies and the conjugates. Ferritin or horseradish peroxidase conjugated goat anti-rabbit IgG was used as a secondary antibody that cross reacted with guinea pig Ig in order to reduce nonspecific immunochemical reactions. Using light microscopy following immunoperoxidase staining, GPCMV antigens in an intranuclear location were not discernable when the infected cells were stained without pretreatment with trypsin, however intranuclear GPCMV antigens could be visualized after the fixed cells were treated with trypsin for 2-4 min prior to addition of the antiserum. EM examination following indirect immunoferritin staining revealed viral antigens localized on viral capsids and on scattered electron dense amorphous matrices but not on the surrounding tubular structures or fibrils. The possibility that tubular structures may be a host cell product produced in response to GPCMV infection is discussed.
