Cytokine mRNA levels and lymphocyte infiltration in pancreatic tissue during experimental chronic pancreatitis induced by dibutyltin dichloride
Cytokine mRNA levels and lymphocyte infiltration in pancreatic tissue during experimental chronic pancreatitis induced by dibutyltin dichloride
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DOI：
10.1023/a:1010689117772
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发表时间：
2001-08-01
影响因子：
3.1
通讯作者：
Emmrich, J
中科院分区：
文献类型：
--
作者：
Sparmann, G;Behrend, S;Emmrich, J
关于炎症细胞在慢性胰腺炎发病机制中的作用，可获得的信息很少。因此，我们分析了慢性胰腺炎大鼠模型中局部细胞因子分布和淋巴细胞的浸润性。用二丁基二氯化锡(DBTC)一次性静脉注射诱导大鼠实验性胰腺炎。在两个月的时间过程中，用竞争性RT-PCR方法观察细胞因子的mRNA表达。用免疫组织化学、流式细胞仪分析和淋巴细胞增殖试验对淋巴细胞进行鉴定。IL-1β、IL-6、IL-5和IL-10在疾病急性期即刻上调，而IL-2、IL-2R和干扰素-γ仅在慢性病程中有淋巴细胞限制性表达。在浸润性淋巴细胞中，以CD4(+)细胞为主，但在慢性过程中CD8(+)细胞增多，导致CD4/CD8比值降低。在慢性炎症过程中，丝裂原诱导的分离的肠系膜淋巴结细胞的激活增加。我们的结果表明，在实验性胰腺炎中，急性炎症反应之后是T淋巴细胞介导的过程。
There is little information available regarding the role of inflammatory cells in the pathogenesis of chronic pancreatitis. Therefore, we analyzed the local cytokine profile and infiltrating lymphocytes in a rat model of chronic pancreatitis. Experimental pancreatitis was induced by a single intravenous application of dibultyltin dichloride (DBTC). During a time course of two months we observed the mRNA expression of cytokines using competitive RT-PCR. Lymphocytes were characterized by immunohistochemistry, FACS analysis, and the lymphocyte proliferation test. IL-1 beta, IL-6, IL-5, and IL-10 were immediately up-regulated in the acute phase of disease, while lymphocyte-restricted expression of IL-2, IL-2R, and IFN-gamma was only found in the chronic course. Among the infiltrating lymphocytes, CD4(+) cells dominated, but during the chronic process there was an increase of CD8(+) cells, resulting in a reduced CD4/CD8 ratio. Mitogen-induced activation of isolated mesenteric lymph node cells increased during the chronic inflammation. Our results suggest that in experimental pancreatitis acute inflammatory reactions are followed by a T-lymphocyte-mediated process.