Antigenic and Receptor Binding Properties of Enterovirus 68
Antigenic and Receptor Binding Properties of Enterovirus 68
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DOI：
10.1128/jvi.03070-13
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发表时间：
2014-03-01
影响因子：
5.4
通讯作者：
Oshitani, Hitoshi
中科院分区：
文献类型：
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作者：
Imamura, Tadatsugu;Okamoto, Michiko;Oshitani, Hitoshi
自1962年首次在下呼吸道感染的儿科患者中检测到肠道病毒68（EV 68）以来，21世纪末，世界不同地区的急性呼吸道感染患者中的EV 68检测率有所增加。然而，这种趋势的潜在分子机制仍然未知。因此，我们的目的是研究近年来检测到的EV 68的抗原性和受体结合特性，并与EV 68的原型菌株Fermon菌株进行比较。我们首先进行中和（NT）和血凝抑制（HI）试验，使用近年来检测到的EV 68株产生的抗血清。我们发现Fermon株的HI和NT滴度比最近检测到的EV 68株低。在最近检测到的EV 68毒株中，不同遗传谱系的毒株之间的HI和NT滴度也存在显著差异。我们使用聚糖阵列分析进一步研究了EV 68菌株对唾液酸寡糖的受体结合特异性。在聚糖阵列分析中，与α 2-3 SA相比，所有测试的EV 68菌株显示对α 2-6连接的唾液酸（α 2-6 SA）的亲和力。我们的研究表明，不同抗原性的菌株的出现是近年来EV 68检出率上升的可能原因。此外，我们发现EV 68优先与α 2-6 SA结合，这表明EV 68可能对上呼吸道有亲和力。重要的是，在21世纪末，世界不同地区的肠道病毒68（EV 68）感染病例数显著增加。我们研究了最近检测到的EV 68菌株的抗原性和受体结合特性，并与EV 68的原型菌株Fermon进行比较。在近年来检测到的EV 68毒株中，不同遗传谱系的毒株之间的血凝抑制（HI）和中和（NT）滴度存在显著差异。我们使用聚糖阵列分析进一步研究了EV 68菌株对唾液酸低聚糖的受体结合特异性，与α 2-3 SA相比，其显示了对α 2 -6连接的唾液酸（α 2-6 SA）的亲和力。本研究提示，不同抗原性毒株的出现可能是近年来EV 68检出率升高的原因。此外，我们揭示了EV 68优选结合α 2-6 SA。这是第一份描述EV 68受体与特定类型唾液酸结合特性的报告。
Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying molecular mechanisms for this trend are still unknown. We therefore aimed to study the antigenicity and receptor binding properties of EV68 detected in recent years in comparison to the prototype strain of EV68, the Fermon strain. We first performed neutralization (NT) and hemagglutination inhibition (HI) tests using antisera generated for EV68 strains detected in recent years. We found that the Fermon strain had lower HI and NT titers than recently detected EV68 strains. The HI and NT titers were also significantly different between strains of different genetic lineages among recently detected EV68 strains. We further studied receptor binding specificities of EV68 strains for sialyloligosaccharides using glycan array analysis. In glycan array analysis, all tested EV68 strains showed affinity for alpha 2-6-linked sialic acids (alpha 2-6 SAs) compared to alpha 2-3 SAs. Our study demonstrates that emergence of strains with different antigenicity is the possible reason for the increased detection of EV68 in recent years. Additionally, we found that EV68 preferably binds to alpha 2-6 SAs, which suggests that EV68 might have affinity for the upper respiratory tract.IMPORTANCENumbers of cases of enterovirus 68 (EV68) infection in different parts of the world increased significantly in the late 2000s. We studied the antigenicity and receptor binding properties of recently detected EV68 strains in comparison to the prototype strain of EV68, Fermon. The hemagglutination inhibition (HI) and neutralization (NT) titers were significantly different between strains of different genetic lineages among recently detected EV68 strains. We further studied receptor binding specificities of EV68 strains for sialyloligosaccharides using glycan array analysis, which showed affinity for alpha 2-6-linked sialic acids (alpha 2-6 SAs) compared to alpha 2-3 SAs. Our study suggested that the emergence of strains with different antigenicities was the possible reason for the increased detections of EV68 in recent years. Additionally, we revealed that EV68 preferably binds to alpha 2-6 SAs. This is the first report describing the properties of EV68 receptor binding to the specific types of sialic acids.