Effect of ceramide N-acyl chain and polar headgroup structure on the properties of ordered lipid domains (lipid rafts)
Effect of ceramide N-acyl chain and polar headgroup structure on the properties of ordered lipid domains (lipid rafts)
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DOI：
10.1016/j.bbamem.2007.05.007
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发表时间：
2007-09-01
影响因子：
3.4
通讯作者：
London, Erwin
中科院分区：
文献类型：
--
作者：
Megha;Sawatzki, Peter;London, Erwin
神经酰胺是一种鞘脂，可以极大地稳定有序的膜结构域（脂筏），并取代其中的胆固醇。富含神经酰胺的筏与多种生物过程有关。由于神经酰胺类似物已用于探测神经酰胺的生物学功能，并可能具有生物医学应用，因此重要的是表征神经酰胺结构如何影响膜特性，包括脂筏稳定性和组成。在这份报告中，荧光猝灭法被用来评估的影响，神经酰胺类似物与不同的N-酰基链或不同的鞘氨醇骨架筏稳定性和甾醇含量。在由SM和二油酰磷脂酰胆碱（DOPC）的1：1（mol：mol）混合物组成的囊泡中，用神经酰胺代替18 mol%的鞘磷脂（SM），有或没有25 mol%的甾醇的效果进行了检查。在不存在甾醇的情况下，富含SM的有序结构域的热稳定性随着神经酰胺N-酰基链长度的增加而增加，其顺序为C-2：0类似于C6：0类似于C8：0
Ceramides are sphingolipids that greatly stabilize ordered membrane domains (lipid rafts), and displace cholesterol from them. Ceramide-rich rafts have been implicated in diverse biological processes. Because ceramide analogues have been useful for probing the biological function of ceramide, and may have biomedical applications, it is important to characterize how ceramide structure affects membrane properties, including lipid raft stability and composition. In this report, fluorescence quenching assays were used to evaluate the effect of analogues of ceramide with different N-acyl chains or different sphingoid backbones on raft stability and sterol content. The effect of replacing 18 mol% of sphingomyelin (SM) with ceramide in vesicles composed of a 1: 1 (mol:mol) mixture of SM and dioleoylphosphatidylcholine (DOPC), with or without 25 mol% sterol, was examined. In the absence of sterol, the thermal stability of the SM-rich ordered domains increased with ceramide N-acyl chain length in the order C-2:0 similar to C6:0 similar to C8:0