Comparative genomic analysis of hyperthermophilic archaeal Fuselloviridae viruses
Comparative genomic analysis of hyperthermophilic archaeal Fuselloviridae viruses
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DOI：
10.1128/jvi.78.4.1954-1961.2004
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发表时间：
2004-02-01
影响因子：
5.4
通讯作者：
Young, M
中科院分区：
文献类型：
--
作者：
Wiedenheft, B;Stedman, K;Young, M
从俄罗斯堪察加和美国黄石国家公园的酸性温泉中分离到的两种梭形病毒(SSV)的全基因组序列已被测定。这些非裂解温带病毒是从超级嗜热的Sulfolobus宿主中分离到的，两种病毒都具有梭形的梭形形态特征，属于梭状病毒科。这两个基因组与之前从日本测得的SSV1基因组和来自冰岛的SSV2基因组相结合，使我们能够对这些地理分布的高温病毒进行系统发育比较。每种病毒都含有一个约15kbp的环状双链DNA基因组，约有34个开放阅读框架(ORF)。这些FuselloVirus ORF与公共数据库中的基因几乎没有相似之处。相反，18个ORF对所有四个分离株都是共同的，可能代表了定义该病毒组的最小基因集。一般来说，基因组一半上的ORF是共线的，高度保守，而另一半上的ORF不是。所有四个基因组中共有的一个ORF是酪氨酸重组酶家族的整合酶。所有四种病毒基因组都整合到它们的宿主tRNA基因中。用于整合的特定tRNA基因各不相同，一个基因组整合成多个基因座。在每个分离物的基因组中都发现了几个独特的ORF。
The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined. These nonlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses share the spindle-shaped morphology characteristic of the Fuselloviridae family. These two genomes, in combination with the previously determined SSV1 genome from Japan and the SSV2 genome from Iceland, have allowed us to carry out a phylogenetic comparison of these geographically distributed hyperthermal viruses. Each virus contains a circular double-stranded DNA genome of similar to15 kbp with approximately 34 open reading frames (ORFs). These Fusellovirus ORFs show little or no similarity to genes in the public databases. In contrast, 18 ORFs are common to all four isolates and may represent the minimal gene set defining this viral group. In general, ORFs on one half of the genome are colinear and highly conserved, while ORFs on the other half are not. One shared ORF among all four genomes is an integrase of the tyrosine recombinase family. All four viral genomes integrate into their host tRNA genes. The specific tRNA gene used for integration varies, and one genome integrates into multiple loci. Several unique ORFs are found in the genome of each isolate.