pmoA Primers for Detection of Anaerobic Methanotrophs
pmoA Primers for Detection of Anaerobic Methanotrophs
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DOI：
10.1128/aem.02960-10
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发表时间：
2011-06-01
影响因子：
4.4
通讯作者：
Ettwig, Katharina F.
中科院分区：
文献类型：
--
作者：
Luesken, Francisca A.;Zhu, Baoli;Ettwig, Katharina F.
已发表的pmoA引物不匹配的pmoA序列的“Methylomirabilis oxyfera”，一种细菌，进行亚硝酸盐依赖性厌氧甲烷氧化。因此，用于检测“Ca.开发了类似Methylomirabilis oxyfera的甲烷氧化菌，并成功地在不同生境的淡水样品中进行了测试。这些引物扩展了现有的分子工具，用于研究环境中的甲烷氧化菌。
Published pmoA primers do not match the pmoA sequence of "Candidatus Methylomirabilis oxyfera," a bacterium that performs nitrite-dependent anaerobic methane oxidation. Therefore, new pmoA primers for the detection of "Ca. Methylomirabilis oxyfera"-like methanotrophs were developed and successfully tested on freshwater samples from different habitats. These primers expand existing molecular tools for the study of methanotrophs in the environment.