Genome-Wide Analysis of MicroRNA Responses to the Phytohormone Abscisic Acid in Populus euphratica.
Genome-Wide Analysis of MicroRNA Responses to the Phytohormone Abscisic Acid in Populus euphratica.
复制标题
胡杨中 MicroRNA 对植物激素脱落酸反应的全基因组分析
DOI：
10.3389/fpls.2016.01184
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发表时间：
2016
影响因子：
5.6
通讯作者：
Yin W
中科院分区：
文献类型：
--
作者：
Duan H;Lu X;Lian C;An Y;Xia X;Yin W
MicroRNA（miRNA）是一类在转录后水平上对植物生长发育和非生物胁迫应答具有调控作用的非编码小RNA。胡杨是木本植物非生物胁迫响应研究的模式树，目前尚未报道胡杨中miRNAs在全基因组水平对植物激素脱落酸（阿坝）的响应。在这里，我们分析了miRNA响应阿坝在全基因组水平在胡杨利用高通量测序。为了系统地进行胡杨中ABA响应性miRNA的全基因组分析，构建了来自三组（对照、用阿坝处理1天和用阿坝处理4天）的九个sRNA文库。每组包括来自三个单独的小植株的三个文库作为生物学复制。总共鉴定了属于75个保守的miRNA家族的151个独特的成熟序列，并且确定了94个独特的序列是新的miRNA，包括56个具有miRNA* 序列的miRNA。在所有的31个保守的miRNAs和31个新的miRNAs响应阿坝显着不同的组。此外，预测了4132个保守和新的miRNAs的靶基因。通过实时定量PCR证实，miRNA的表达变化与其推定靶点的表达谱呈负相关。杨树特有的或新的miRNA-靶标相互作用可能参与了一些与抗逆性相关的生物学过程。我们的分析提供了一个全面的观点，胡杨miRNA如何响应阿坝，此外，不同的时间动态观察ABA处理的文库。
MicroRNA (miRNA) is a type of non-coding small RNA with a regulatory function at the posttranscriptional level in plant growth development and in response to abiotic stress. Previous studies have not reported on miRNAs responses to the phytohormone abscisic acid (ABA) at a genome-wide level in Populus euphratica, a model tree for studying abiotic stress responses in woody plants. Here we analyzed the miRNA response to ABA at a genome-wide level in P. euphratica utilizing high-throughput sequencing. To systematically perform a genome-wide analysis of ABA-responsive miRNAs in P. euphratica, nine sRNA libraries derived from three groups (control, treated with ABA for 1 day and treated with ABA for 4 days) were constructed. Each group included three libraries from three individual plantlets as biological replicate. In total, 151 unique mature sequences belonging to 75 conserved miRNA families were identified, and 94 unique sequences were determined to be novel miRNAs, including 56 miRNAs with miRNA* sequences. In all, 31 conserved miRNAs and 31 novel miRNAs response to ABA significantly differed among the groups. In addition, 4132 target genes were predicted for the conserved and novel miRNAs. Confirmed by real-time qPCR, expression changes of miRNAs were inversely correlated with the expression profiles of their putative targets. The Populus special or novel miRNA-target interactions were predicted might be involved in some biological process related stress tolerance. Our analysis provides a comprehensive view of how P. euphratica miRNA respond to ABA, and moreover, different temporal dynamics were observed in different ABA-treated libraries.