Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms
Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms
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DOI：
10.1002/pro.5560070420
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发表时间：
1998-04-01
期刊：
影响因子：
8
通讯作者：
von Heijne, G
中科院分区：
文献类型：
--
作者：
Wallin, E;von Heijne, G
我们已经进行了详细的统计分析，从真细菌，古细菌和真核生物的全基因组序列数据的螺旋束类的完整膜蛋白。所有ORF的20%至30%被预测为编码膜蛋白，较大的基因组比较小的基因组含有更高的分数。虽然有一个普遍的趋势，即具有较少数量的跨膜片段的蛋白质比那些有很多，单细胞生物似乎更喜欢的蛋白质与6和12个跨膜片段，而秀丽隐杆线虫和智人有轻微的偏好与7个跨膜片段的蛋白质。在所有生物体中，存在这样的趋势，即膜蛋白要么具有许多具有短连接环的跨膜区段，要么具有很少的具有大膜外结构域的跨膜区段。来自所研究的所有生物体的膜蛋白，可能除了古菌詹氏甲烷球菌之外，都遵循所谓的“阳性内部”规则;即，它们倾向于在细胞质中比在细胞质外片段中具有更高频率的带正电荷的残基。
We have carried out detailed statistical analyses of integral membrane proteins of the helix-bundle class from eubacterial, archaean, and eukaryotic organisms for which genome-wide sequence data are available. Twenty to 30% of all ORFs are predicted to encode membrane proteins, with the larger genomes containing a higher fraction than the smaller ones. Although there is a general tendency that proteins with a smaller number of transmembrane segments are more prevalent than those with many, uni-cellular organisms appear to prefer proteins with 6 and 12 transmembrane segments, whereas Caenorhabditis elegans and Homo sapiens have a slight preference for proteins with seven transmembrane segments. In all organisms, there is a tendency that membrane proteins either have many transmembrane segments with short connecting loops or few transmembrane segments with large extra-membraneous domains. Membrane proteins from all organisms studied, except possibly the archaeon Methanococcus jannaschii, follow the so-called "positive-inside" rule; i.e., they tend to have a higher frequency of positively charged residues in cytoplasmic than in extracytoplasmic segments.