Electron capture dissociation and 13C, 15N depletion for deuterium localization in intact proteins after solution-phase exchange
Electron capture dissociation and 13C, 15N depletion for deuterium localization in intact proteins after solution-phase exchange
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DOI：
10.1021/ac020690k
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发表时间：
2003-07-01
影响因子：
7.4
通讯作者：
Kelleher, NL
中科院分区：
文献类型：
--
作者：
Charlebois, JP;Patrie, SM;Kelleher, NL
为了在与D2O溶液交换后定位氘原子，通常在质谱（MS）和串联质谱（MS/MS）分析之前先消化完整的蛋白质。改进与此方法相关的局限性(例如：
For localization of deuterium atoms after solution-phase exchange with D2O, intact proteins are often digested prior to analysis by mass spectrometry (MS) and tandem MS (MS/MS). Amelioration of limitations associated with this approach (e.g.,