Whole-genome analysis of mRNA decay in Plasmodium falciparum reveals a global lengthening of mRNA half-life during the intra-erythrocytic development cycle.
Whole-genome analysis of mRNA decay in Plasmodium falciparum reveals a global lengthening of mRNA half-life during the intra-erythrocytic development cycle.
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DOI：
10.1186/gb-2007-8-7-r134
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
DeRisi JL
中科院分区：
文献类型：
--
作者：
Shock JL;Fischer KF;DeRisi JL
树和解方法中的一致的偏见，发生在推断的基因树是不正确的，怀疑以前的结论古代复制和损失的脊椎动物基因组的历史。mRNA的降解速率是所有生物体中转录后调控的基本要素。以前，在几种生物体中的研究发现，每种mRNA的特定半衰期与其生理作用精确相关，并在决定基因表达水平方面发挥重要作用。我们使用了全基因组的方法来表征恶性疟原虫的mRNA衰变。我们发现，在全球范围内，在无性红细胞内发育周期的mRNA衰变率显着增加。在环阶段的周期，平均mRNA的半衰期为9.5分钟，但这是延长到平均65分钟，在后期的发育阶段。因此，mRNA衰减率的主要决定因素似乎与红细胞内发育阶段有关。此外，我们发现特定的变化，衰变模式叠加在主导趋势的渐进半衰期延长。这些衰变模式的变化经常富集具有特定细胞功能或过程的基因。阐明疟原虫的mRNA衰变率提供了一个关键因素，破译这种寄生虫的遗传控制机制，通过补充和扩展以前的mRNA丰度研究。我们的研究结果表明，渐进的阶段依赖性下降的mRNA衰减率功能是一个主要的决定因素的mRNA积累在红细胞内发育的前体阶段。迄今为止，在任何其他生物体中均未观察到这种mRNA衰减速率的全基因组变化，这表明转录后调控可能是恶性疟原虫基因调控的主导机制。
A consistent bias in tree reconciliation methods is described that occurs when the inferred gene tree is not correct, casting doubt on previous conclusions about ancient duplications and losses in vertebrate genome history. The rate of mRNA decay is an essential element of post-transcriptional regulation in all organisms. Previously, studies in several organisms found that the specific half-life of each mRNA is precisely related to its physiologic role, and plays an important role in determining levels of gene expression. We used a genome-wide approach to characterize mRNA decay in Plasmodium falciparum. We found that, globally, rates of mRNA decay increase dramatically during the asexual intra-erythrocytic developmental cycle. During the ring stage of the cycle, the average mRNA half-life was 9.5 min, but this was extended to an average of 65 min during the late schizont stage of development. Thus, a major determinant of mRNA decay rate appears to be linked to the stage of intra-erythrocytic development. Furthermore, we found specific variations in decay patterns superimposed upon the dominant trend of progressive half-life lengthening. These variations in decay pattern were frequently enriched for genes with specific cellular functions or processes. Elucidation of Plasmodium mRNA decay rates provides a key element for deciphering mechanisms of genetic control in this parasite, by complementing and extending previous mRNA abundance studies. Our results indicate that progressive stage-dependent decreases in mRNA decay rate function are a major determinant of mRNA accumulation during the schizont stage of intra-erythrocytic development. This type of genome-wide change in mRNA decay rate has not been observed in any other organism to date, and indicates that post-transcriptional regulation may be the dominant mechanism of gene regulation in P. falciparum.
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影响因子：
9.8
作者：
Bozdech, Zbynek;Llinas, Manuel;Pulliam, Brian Lee;Wong, Edith D;Zhu, Jingchun;DeRisi, Joseph L
通讯作者：
DeRisi, Joseph L
影响因子：
10.5
作者：
Lotan, R;Bar-On, VG;Choder, M
通讯作者：
Choder, M
影响因子：
7
作者：
Coulson, RMR;Hall, N;Ouzounis, CA
通讯作者：
Ouzounis, CA
微阵列数据中词汇偏差的显着性分析。
影响因子：
3
作者：
Kim CC;Falkow S
通讯作者：
Falkow S
DOI：
10.1016/0035-9203(85)90119-1
发表时间：
1985-01-01
影响因子：
2.2
作者：
DEROJAS, MO;WASSERMAN, M
通讯作者：
WASSERMAN, M