Screening and Functional Analyses of Nilaparvata lugens Salivary Proteome
Screening and Functional Analyses of Nilaparvata lugens Salivary Proteome
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褐飞虱唾液蛋白质组的筛选及功能分析
DOI：
10.1021/acs.jproteome.6b00086
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发表时间：
2016-06-01
影响因子：
4.4
通讯作者：
Bao, Yan-Yuan
中科院分区：
文献类型：
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作者：
Huang, Hai-Jian;Liu, Cheng-Wen;Bao, Yan-Yuan
大多数以韧皮部为食的昆虫在进食过程中分泌胶凝和水状唾液。然而，唾液蛋白的功能知之甚少。本研究旨在揭示水稻吸汁害虫褐飞虱唾液的组成和功能。N.全基因组和转录组测序的完成。lugens的表达有助于阐明唾液蛋白的基因信息和表达特异性。在这项研究中，我们已经，第一次，确定了丰富的蛋白质组分从胶凝和水唾液中的单食性吸汁昆虫物种通过鸟枪蛋白质组学检测结合基因组和转录组学分析。8个未知的分泌蛋白仅限于N.褐色，表明物种特异性唾液成分。在分泌唾液中发现的一组膜联蛋白样蛋白具有与典型的昆虫膜联蛋白不同的结构域和表达特异性。编码5种膜联蛋白样蛋白、6种唾液蛋白（功能未知的唾液腺特异性蛋白）、7种推定酶和1种粘蛋白样蛋白的19个基因表现出唾液腺特异性表达模式，表明它们在唾液腺和唾液的生理机制中的重要性该昆虫物种。RNA干扰结果表明，salivap-3是唾液鞘形成的关键蛋白因子，而annexin-like 5和碳酸酐酶是N. lugens生存。这些新的发现将有助于阐明唾液蛋白在N. lugens之间的相互作用机制。褐飞虱与水稻的关系，为今后水稻害虫的防治提供了重要的靶标。
Most phloem-feeding insects secrete gelling and watery saliva during the feeding process. However, the functions of salivary proteins are poorly understood. In this study, our purpose was to reveal the components and functions of saliva in a rice sap-sucking insect pest, Nilaparvata lugens. The accomplishment of the whole genome and transcriptome sequencing in N. lugens would be helpful for elucidating the gene information and expression specificity of the salivary proteins. In this study, we have, for the first time, identified the abundant protein components from gelling and watery saliva in a monophagous sap-sucking insect species through shotgun proteomic detection combined with the genomic and transcriptomic analysis. Eight unknown secreted proteins were limited to N. lugens, indicating species-specific saliva components. A group of annexin-like proteins first identified in the secreted saliva displayed different domain structure and expression specificity with typical insect annexins. Nineteen genes encoding five annexin-like proteins, six salivaps (salivary glands-specific proteins with unknown function), seven putative enzymes, and a mucin-like protein showed salivary gland specific expression pattern, suggesting their importance in the physiological mechanisms of salivary gland and saliva in this insect species. RNA interference revealed that salivap-3 is a key protein factor in forming the salivary sheath, while annexin-like5 and carbonic anhydrase are indispensable for N. lugens survival. These novel findings will greatly help to clarify the detailed functions of salivary proteins in the physiological process of N. lugens and elucidate the interaction mechanisms between N. lugens and the rice plant, which could- provide important targets for the future management of rice pests.