Accelerated Microparticulate Bed Liquid Chromatography.
Accelerated Microparticulate Bed Liquid Chromatography.
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加速微粒床液相色谱。
DOI：
10.1021/ac60236a048
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发表时间：
1966
影响因子：
7.4
通讯作者：
E. Piel
中科院分区：
文献类型：
--
作者：
E. Piel
纸色谱和薄层色谱的离心发展使纸色谱和薄层色谱的速度大大提高（1）。在过去，接近大气压的真空和压差被用于加速柱色谱（2）。然而，为了保持合理的展开时间，目前的柱色谱实践涉及使用平均直径至少约25微米的床颗粒。已经发现两种不同的迫使液体通过微粒床的方法可以提高柱色谱的速度，并在分辨率、吸附能力和处理微量或宏观色谱问题的能力方面产生优势。
CENTRIFUGAL DEVELOPMENT of paper chromatograms and of thin layer chromatograms has enabled considerable increase in speed for paper and thin layer chromatography (1). Vacuum and pressure differentials approaching an atmosphere have, in the past, been used to accelerate column chromatography {2). Nevertheless, in orderto maintain reasonable development times, present column chromatography practice in-volves use of bed particles of at least about 25 microns average diameter. Two different methods for forcing liquids through microparticulate beds have been foundto increase speed for column chromatography, and to produce advantages in resolution, adsorbent capacity, and handling ability formicro or macro chromatographic problems.