An analysis using the hobo genetic system reveals that combinatorial signaling by the Dpp and Wg pathways regulates dpp expression in leading edge cells of the dorsal ectoderm in Drosophila melanogaster.
An analysis using the hobo genetic system reveals that combinatorial signaling by the Dpp and Wg pathways regulates dpp expression in leading edge cells of the dorsal ectoderm in Drosophila melanogaster.
复制标题
使用 Hobo 遗传系统进行的分析表明，Dpp 和 Wg 途径的组合信号传导可调节果蝇背侧外胚层前缘细胞中的 dpp 表达。
DOI：
10.1093/genetics/161.2.685
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发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Takaesu,NT
中科院分区：
文献类型：
--
作者：
Newfeld,SJ;Takaesu,NT
Our laboratory has contributed to the development of a genetic system based upon thehobotransposable element inDrosophila melanogaster. We recently reported thathobo, like the better-knownPelement, is capable of local transposition. In that study, we mobilized ahoboenhancer trap vector and generated two unique alleles ofdecapentaplegic(dpp), a transforming growth factor-β family member with numerous roles during development. Here we report a detailed study of one of those alleles (dppF11). To our knowledge, this is the first application of thehobogenetic system to understanding developmental processes. First, we demonstrate that lacZ expression from thedppF11enhancer trap accurately reflectsdppmRNA accumulation in leading edge cells of the dorsal ectoderm. Then we show that combinatorial signaling by the Wingless (Wg) pathway, the Dpp pathway, and the transcriptional coactivator Nejire (CBP/p300) regulatesdppF11expression in these cells. Our analysis ofdppF11suggests a model for the integration of Wg and Dpp signals that may be applicable to other developmental systems. Our analysis also illustrates several new features of thehobogenetic system and highlights the value ofhobo, as an alternative toP, in addressing developmental questions.
