Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering
Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering
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DOI：
10.1002/jbm.a.36754
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发表时间：
2019-07-22
影响因子：
4.9
通讯作者：
Douglas, Timothy E. L.
中科院分区：
文献类型：
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作者：
Dziadek, Michal;Kudlackova, Radmila;Douglas, Timothy E. L.
本工作的重点是开发新型的多组分有机-无机水凝胶复合材料的骨组织工程。首次提出了食品工业中常用的有机组分，即乳清蛋白分离物（WPI）和来自牛皮的明胶，以及通常用作水硬性骨水泥的主要组分的无机材料，即各种浓度（0-70重量%）的α-TCP的组合。结果表明，在水凝胶制备过程中，α-TCP不完全转化为缺钙羟基磷灰石（CDHA）。计算机断层扫描显示磷酸钙（CaP）相在所得复合材料中的不均匀分布。然而，含有30-70重量%的α-TCP的水凝胶显示出显著改善的机械性能。杨氏模量和对应于样品压缩50%的应力的值随着陶瓷相浓度的增加几乎线性地增加。在复合材料的制备过程中，α-TCP向CDHA的不完全转化使其在模拟体液中在14天的孵育期间具有高反应性。初步的体外研究表明，WPI/明胶/CaP复合水凝胶支持人成骨样MG-63细胞的粘附、铺展和增殖。在这项工作中获得的WPI/明胶/CaP复合水凝胶显示出巨大的潜力，用于骨组织工程和再生医学的应用。
The present work focuses on the development of novel multicomponent organic-inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely alpha-TCP in various concentrations (0-70 wt%) was proposed. The results showed that alpha-TCP underwent incomplete transformation to calcium-deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30-70 wt% alpha-TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of alpha-TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14-day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast-like MG-63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications.