Demonstration of a specific receptor for prolactin in porcine granulosa cells.
Demonstration of a specific receptor for prolactin in porcine granulosa cells.
复制标题
猪颗粒细胞中催乳素特异性受体的演示。
DOI：
10.3109/07435807509053855
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
J. Hammond
中科院分区：
文献类型：
--
作者：
R. Rolland;J. Hammond
猪颗粒细胞和这些细胞的亚细胞组分已被证明具有绵羊催乳素（OPRL）的特异性受体。绵羊生长激素在从其结合位点置换125 I-OPRL方面的效力为OPRL的7%; FSH、TSH、LH、胰岛素和ACTH的交叉反应性可忽略不计。Scatchard曲线分析表明，~（125）I-OPRL对颗粒细胞受体有很高的亲和力，其解离常数（Kd）为7.4-7.7 × 10 ~（-10）M，随卵泡增大而无变化。相反，催乳素的特异性结合随着卵泡的成熟而显著降低，结合位点/细胞从小卵泡的555个明显减少到大卵泡（排卵前）的300个。所证明的Kd值在体内催乳素浓度范围内，并且与我们实验室和其他地方获得的乳腺和其他组织催乳素受体的值非常一致。因此，这些研究可能为更好地了解催乳素在卵巢功能中的作用提供了基础。
Porcine granulosa cells and subcellular fractions from these cells have been shown to have a specific receptor for ovine prolactin (OPRL). Ovine growth hormone demonstrated 7% of the potency of OPRL in displacing 125I-OPRL from its binding site; FSH, TSH, LH, insulin and ACTH showed negligible cross-reactivity. Scatchard analysis of the displacement curves suggested that 125I-OPRL has a high affinity for its receptor with a dissociation constant (Kd) for the granulosa cell-receptor of 7.4-7.7 times 10(-10) M with no change as the follicle enlarges. In contrast, the specific binding of prolactin decreased markedly with maturation of the follicles with an apparent decrease in binding sites/cell from 555 in small follicles to 300 in large (preovulatory) follicles. The demonstrated Kd's were within the range of prolactin concentrations easily attained in vivo and were in good agreement with values obtained in our laboratory and elsewhere for the prolactin receptor from mammary gland and other tissues. Consequently, these studies may provide a basis for a better understanding of the role of prolactin in ovarian function.