Physiological characterization of the cold-shock-induced humoral factor for wing color-pattern changes in butterflies
Physiological characterization of the cold-shock-induced humoral factor for wing color-pattern changes in butterflies
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DOI：
10.1016/j.jinsphys.2010.02.013
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发表时间：
2010-09-01
影响因子：
2.2
通讯作者：
Otaki, Joji M.
中科院分区：
文献类型：
--
作者：
Mahdi, Shah H. A.;Gima, Shinichi;Otaki, Joji M.
在化蛹后立即对蛹施加温度休克可以改变蝴蝶翅膀的颜色模式，这归因于冷休克诱导的体液因子，称为冷休克激素（CSH）。在这里，我们的生理特点CSH和药理作用的钨酸盐，使用一个若虫蝴蝶Junonia orithya。我们首先证明了精确的修饰模式依赖于化蛹后冷休克处理的时间点，并证实了在冷休克蛹中诱导的修饰特性能够在共生实验中转移到另一个蛹中。头和前胸一起切除后的冷休克应用程序仍然产生修改的翅膀，不包括脑后神经内分泌复合体的主要参与。此外，钨酸盐注射诱导修改，即使在个人的头部和前胸被删除。重要的是，移植从冷休克蛹分离的气管诱导受体翅膀的修改。我们确定了一个化学峰，在血淋巴中的冷休克的个人使用HPLC，这对应于多巴胺，并证明多巴胺及其相关的生物胺有能力引起小的颜色模式的变化。两者合计，本研究表明，CSH可能是分泌气管相关的内分泌细胞后，冷休克治疗和钨酸盐可能会改变颜色模式，通过其直接作用于翅膀。(C)2010爱思唯尔有限公司版权所有。
Butterfly wing color patterns can be modified by the application of temperature shock to pupae immediately after pupation, which has been attributed to a cold-shock-induced humoral factor called cold-shock hormone (CSH). Here, we physiologically characterized CSH and pharmacological action of tungstate, using a nymphalid butterfly Junonia orithya. We first showed that the precise patterns of modification were dependent on the time-point of the cold-shock treatment after pupation, and confirmed that the modification properties induced in a cold-shocked pupa were able to be transferred to another pupa in a parabiosis experiment. Cold-shock application after removal of the head and prothorax together still produced modified wings, excluding major involvement of the brain-retrocerebral neuroendocrine complex. Furthermore, tungstate injection induced modifications even in individuals whose head and prothorax were removed. Importantly, transplantation of tracheae isolated from cold-shocked pupae induced modifications in the recipient wings. We identified a chemical peak in hemolymph of the cold-shocked individuals using HPLC, which corresponded to dopamine, and demonstrated that dopamine and its related biogenic amines have ability to induce small color-pattern changes. Taken together, the present study suggests that CSH is likely to be secreted from trachea-associated endocrine cells upon cold-shock treatment and that tungstate may change color patterns via its direct action on wings. (C) 2010 Elsevier Ltd. All rights reserved.