Lytic activity of recombinant bacteriophage φ11 and φ12 endolysins on whole cells and biofilms of Staphylococcus aureus
Lytic activity of recombinant bacteriophage φ11 and φ12 endolysins on whole cells and biofilms of Staphylococcus aureus
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DOI：
10.1128/aem.01616-06
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Bierbaum, Gabriele
中科院分区：
文献类型：
--
作者：
Sass, Peter;Bierbaum, Gabriele
重组phi 11内溶素水解热灭活葡萄球菌以及葡萄球菌生物膜。细胞壁靶向似乎是裂解整个细胞的先决条件，并且内肽酶和酰胺酶结构域的组合作用是最大活性所必需的。相比之下，phi 12内溶素是无活性的并引起细胞聚集。
The recombinant phi 11 endolysin hydrolyzed heat-killed staphylococci as well as staphylococcal biofilms. Cell wall targeting appeared to be a prerequisite for lysis of whole cells, and the combined action of the endopeptidase and amidase domains was necessary for maximum activity. In contrast, the phi 12 endolysin was inactive and caused aggregation of the cells.