Screening of pea (Pisum sativum L.) genotypes for salt tolerance based on early growth stage attributes and leaf inorganic osmolytes.
Screening of pea (Pisum sativum L.) genotypes for salt tolerance based on early growth stage attributes and leaf inorganic osmolytes.
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发表时间：
2012-09
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通讯作者：
M. Shahid;M. A. Pervez;R. Balal;T. Abbas;C. M. Ayyub;N. Mattson;A. Riaz;Z. Iqbal
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作者：
M. Shahid;M. A. Pervez;R. Balal;T. Abbas;C. M. Ayyub;N. Mattson;A. Riaz;Z. Iqbal
为了探讨测定豌豆生长前期对盐胁迫敏感性的筛选指标，在以细沙为生长介质的托盘上，用0、2.5、5.0、7.5和10dsm-1NaC l 5个盐度水平对30个豌豆品种(Pisum Sativum)进行了培养。以Hoagland‘s液作为基础营养液。分别在培养皿和塑料托盘中观察发芽率和出苗率。盐胁迫显著降低了发芽率、出苗率、根冠鲜重、根冠干重、根冠长和叶片无机渗透压(K+、Ca~(2+))。在盐分条件下，所有品种的叶片Na+含量均增加，但耐性品种的叶片Na+含量最低。叶片Na+积累量高表明对盐害特别敏感，而叶片K+/Na+和Ca~(2+)/Na+比值高则表明叶片伤害程度较低。与对照相比，高盐胁迫(7.5和10dsm-1)使供试品种的上述性状发生了较大的变化。根/冠干重与叶片K+/Na+、Ca~(2+)/Na~+比值呈显著正相关。根据测定指标的增减百分比，供试豌豆品种在苗期可分为耐盐性较弱、耐盐性中等和耐盐性较高的三类。
In order to investigate the screening attributes that can be used to determine early growth stage sensitivity of pea plants to salt stress, 30 pea genotypes (Pisum sativum) were grown in trays containing fine sand as growth medium and subjected to five different salinity levels, i.e. 0, 2.5, 5.0, 7.5 and 10 dS m-1 NaCl. The Hoagland's solution was applied as the base nutrient solution. The germination (%) and emergence (%) was investigated in Petri dishes and plastic trays, respectively. Salt stress significantly reduced the germination (%), emergence (%), root and shoot fresh weight, root and shoot dry weight, root and shoot length and leaf inorganic osmolytes (K+ and Ca2+). While the leaf Na+ content increased in all the genotypes, tolerant genotypes exhibited the lowest leaf Na+ content under saline conditions. High leaf Na+ accumulation indicated genotypes which are particularly sensitive to salt damage while high leaf K+/Na+ and Ca2+/Na+ ratio indicated genotypes with lower leaf injury. Compared with control, all added salinity levels induced an injurious effect on the above mentioned attributes but high salt stress (7.5 and 10 dS m-1) resulted in considerable variation in tested genotype. A significant correlation was found between the root/shoot dry weight and leaf K+/Na+ and Ca2+/Na+ ratios. Based on the percent increase or decrease over control for the measured attributes, the tested pea genotypes were categorized into less salt tolerant, intermediate and high salt tolerant groups at seedling stage.