COMPARISON OF SHORT-TERM INDIRECT CALORIMETRY AND DOUBLY LABELED WATER METHOD FOR THE ASSESSMENT OF ENERGY-EXPENDITURE IN PRETERM INFANTS
COMPARISON OF SHORT-TERM INDIRECT CALORIMETRY AND DOUBLY LABELED WATER METHOD FOR THE ASSESSMENT OF ENERGY-EXPENDITURE IN PRETERM INFANTS
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DOI：
10.1159/000243391
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发表时间：
1991-08-01
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影响因子：
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通讯作者：
SAUER, PJJ
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作者：
WESTERTERP, KR;LAFEBER, HN;SAUER, PJJ
在8名健康早产儿（出生体重1，270 +/- 193 g，胎龄32 +/- 3周，平均值+/- SD）中研究了8小时间接热量测定法（IDC）作为能量消耗估计值的准确性，并与使用双标记水（H2O）-H2-O-18方法（DLW）进行的5天分析进行了比较。 连续鼻饲120 kcal/kg/d婴儿专用配方奶粉的婴儿，在密闭系统间接热量计中于8h内进行两次热中性测量，间隔4d;同时，通过同位素比质谱法测量在口服（H2O）-H2-O-后6小时每天收集的尿样中的同位素衰变。18在IDC的第一天，都在出生后第4周。 使用两点分析或多点分析，通过单次8小时IDC或重复8小时IDC和5天DLW方法测量的二氧化碳产生率（rCO 2）之间的平均差异与零无显著差异。 使用两点分析的DLW方法计算的rCO 2与多点分析测量的rCO 2相差-1.4 +/- 1.7%。 根据IDC 8小时和5天DLW方法（基于实测RQ为0.90）估计的代谢率之间的平均差异为-6.7 +/- 6.2%，根据饲喂RQ估计的代谢率之间的平均差异为-4.5 +/-6.0%。 这些差异与零无显著差异。 我们的结论是，IDC超过8小时和两点DLW测量超过5天，这两种方法，可以在实践中应用相对容易，提供了一个足够的平均估计的能量消耗在连续喂养的早产儿在热中性条件下。
The accuracy of 8-hour indirect calorimetry (IDC) as an estimate of energy expenditure was investigated in 8 healthy preterm infants (birth weight 1,270 +/- 193 g, gestational age 32 +/- 3 weeks, mean +/- SD) in comparison with an analysis over 5 days using the doubly-labeled water (H2O)-H-2-O-18 method (DLW). The infants that were fed continuously by nasogastric drip with 120 kcal/kg/day of special infant formula were measured twice under thermoneutral conditions in a closed system indirect calorimeter during 8 h with a 4-day interval; simultaneously isotope decay was measured by isotope ratio mass spectrometry in urine samples collected daily during 5 days from 6 h after an oral dose of (H2O)-H-2-O-18 on the first day of IDC, all during the 4th postnatal week. The mean differences between carbon dioxide production rate (rCO2) measured either by single 8-hour IDC or by duplicate 8-hour IDC and the 5-day DLW method, using the two-point analysis or the multipoint analysis were not significantly different from zero. The rCO2 calculated from the DLW method using the two-point analysis differed -1.4 +/- 1.7% from that measured by the multipoint analysis. The mean differences between the metabolic rate estimated from 8 h of IDC and from the 5-day DLW method based on a measured RQ of 0.90 was -6.7 +/- 6.2% and based on the RQ of the feeding -4.5 +/- 6.0%. These differences were not significantly different from zero. We conclude that IDC over 8 h and two-point DLW measurement over 5 days, both methods that can be applied with relative ease in practice, offer an adequate average estimate of energy expenditure in continuously fed preterm infants under thermoneutral conditions.