The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites
The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites
复制标题
TCTP直系同源基因AmphiTCTP在文昌鱼中与脊索和体节发育相关的表达
DOI：
10.1016/j.cbpb.2007.02.012
复制
发表时间：
2007-07-01
影响因子：
2.2
通讯作者：
Zhang, Peijun
中科院分区：
文献类型：
--
作者：
Chen, Zhongke;Zhang, Hongwei;Zhang, Peijun
肿瘤抑制蛋白（TCTP）是一种高度保守的蛋白质，广泛存在于真核生物中。本文报道了头索动物文昌鱼TCTP同源基因AmphiTCTP的系统发育分析和发育表达。系统进化分析表明，AmphiTCTP的推定蛋白质与脊椎动物的同源物接近。原位杂交和RT-PCR检测结果表明，AmphiTCTP在受精卵、卵裂早期胚胎和大部分早期发育阶段均有表达，但在卵裂晚期至原肠胚中期未检测到AmphiTCTP的表达。AmphiTCTP在受精卵和卵裂早期的表达表明AmphiTCTP可能是一个母系基因。从早期的神经胚阶段向前，AmphiTCTP转录本是本地化的推定脊索，presomitic中胚层，和新生体节。然而，在脊索和体节形成后，其表达逐渐下调。AmphiTCTP的表达模式与脊索和体节的分化相一致，提示AmphiTCTP可能不是管家基因，而是在中胚层发育中起重要作用。(c)2007年爱思唯尔公司All rights reserved.
The translationally controlled tumor protein (TCTP) is highly conserved and has been widely found in eukaryotic organisms. Here, we report the phylogenetic analysis and developmental expression of AmphiTCTP, a TCTP homologous gene in cephalochordate amphioxus. Phylogenetic analysis indicates that the putative protein of AmphiTCTP is close to its vertebrate orthologs. The mRNA of AmphiTCTP is found in fertilized eggs, early cleavage embryo and most of the early developmental stages by in situ hybridization and RT-PCR, but its expression is not detectable from late cleavage stage to mid-gastrula. The expression of AmphiTCTP in zygotes and early cleavage stages shows that AmphiTCTP may be a maternal gene. From the early neurula stage onward, AmphiTCTP transcript is localized in the presumptive notochord, presomitic mesoderm, and nascent somites. However, its expression is gradually down-regulated after the notochord and somites have been formed. The expression pattern of AmphiTCTP thus coincides with the differentiation of the notochord and somites, this suggests that AmphiTCTP may not be a housekeeping gene and may play an important role in mesoderm development. (c) 2007 Elsevier Inc. All rights reserved.