Genome-wide Identification of WRKY Genes in the Desert Poplar Populus euphratica and Adaptive Evolution of the Genes in Response to Salt Stress.
Genome-wide Identification of WRKY Genes in the Desert Poplar Populus euphratica and Adaptive Evolution of the Genes in Response to Salt Stress.
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DOI：
10.4137/ebo.s22067
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Ma J;Lu J;Xu J;Duan B;He X;Liu J
WRKY转录因子在植物的发育和对各种逆境胁迫的应答中起着重要的作用。然而，对于高度耐盐胁迫的沙漠白杨物种胡杨树中WRKY基因的进化知之甚少。在这项研究中，我们从胡杨基因组中鉴定了107个PeWRKY基因，并研究了它们与盐敏感同源物大果杨WRKY基因的进化关系。10个PeWRKY基因是胡杨特有的，其中5个在盐胁迫下表达改变。此外，我们发现两个物种之间的两对直系同源物显示出正向进化的证据，dN/dS比值>1（非同义/同义替换），并且它们都改变了它们的表达以响应盐胁迫。这些结果表明，新基因的开发和WRKY基因家族中某些同源基因的正向进化可能在胡杨获得高耐盐性中发挥了重要作用。
WRKY transcription factors play important roles in plant development and responses to various stresses in plants. However, little is known about the evolution of the WRKY genes in the desert poplar species Populus euphratica, which is highly tolerant of salt stress. In this study, we identified 107 PeWRKY genes from the P. euphratica genome and examined their evolutionary relationships with the WRKY genes of the salt-sensitive congener Populus trichocarpa. Ten PeWRKY genes are specific to P. euphratica, and five of these showed altered expression under salt stress. Furthermore, we found that two pairs of orthologs between the two species showed evidence of positive evolution, with dN/dS ratios>1 (nonsynonymous/synonymous substitutions), and both of them altered their expression in response to salinity stress. These findings suggested that both the development of new genes and positive evolution in some orthologs of the WRKY gene family may have played an important role in the acquisition of high salt tolerance by P. euphratica.