Does calorie restriction induce mitochondrial biogenesis? A reevaluation
Does calorie restriction induce mitochondrial biogenesis? A reevaluation
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DOI：
10.1096/fj.10-170415
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发表时间：
2011-02-01
期刊：
影响因子：
4.8
通讯作者：
Holloszy, John O.
中科院分区：
文献类型：
--
作者：
Hancock, Chad R.;Han, Dong-Ho;Holloszy, John O.
据报道，30%卡路里限制（CR）3个月导致心脏、脑、肝脏和脂肪组织中线粒体生物合成大幅增加，同时呼吸和ATP合成增加。我们发现这些结果令人惊讶，并进行了这项研究，以确定30%CR是否会诱导心脏，大脑，肝脏，脂肪组织和/或骨骼肌中线粒体的增加。为此，我们测量了一系列线粒体蛋白和mRNA的水平。除了长链酰基辅酶A脱氢酶蛋白水平，这是增加了类似于60%的脂肪组织中，没有线粒体蛋白或mRNA，我们测量的增加在大鼠进行30%CR 14周。柠檬酸合酶活性也没有增加。由于在没有关键线粒体蛋白质增加的情况下线粒体增加是不可能的，因此我们得出结论，30% CR不会诱导实验室啮齿动物心脏，大脑，肝脏，脂肪组织或骨骼肌中线粒体的增加。汉考克角R.，汉，D.- H、Higashida，K.，Kim，S. H、霍洛西限制热量摄入能诱导线粒体生物合成吗？重新评估FASEB J.25，785-791（2011）。www.fasebj.org
It has been reported that 30% calorie restriction (CR) for 3 mo results in large increases in mitochondrial biogenesis in heart, brain, liver, and adipose tissue, with concomitant increases in respiration and ATP synthesis. We found these results surprising, and performed this study to determine whether 30% CR does induce an increase in mitochondria in heart, brain, liver, adipose tissue, and/or skeletal muscle. To this end, we measured the levels of a range of mitochondrial proteins, and mRNAs. With the exception of long-chain acyl-CoA dehydrogenase protein level, which was increased similar to 60% in adipose tissue, none of the mitochondrial proteins or mRNAs that we measured were increased in rats subjected to 30% CR for 14 wk. There was also no increase in citrate synthase activity. Because it is not possible to have an increase in mitochondria without any increase in key mitochondrial proteins, we conclude that 30% CR does not induce an increase in mitochondria in heart, brain, liver, adipose tissue, or skeletal muscle in laboratory rodents.-Hancock, C. R., Han, D.-H., Higashida, K., Kim, S. H., Holloszy, J. O. Does calorie restriction induce mitochondrial biogenesis? A reevaluation. FASEB J. 25, 785-791 (2011). www.fasebj.org