A facile microfluidic paper-based analytical device for acetylcholinesterase inhibition assay utilizing organic solvent extraction in rapid detection of pesticide residues in food
A facile microfluidic paper-based analytical device for acetylcholinesterase inhibition assay utilizing organic solvent extraction in rapid detection of pesticide residues in food
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一种简便的微流体纸基分析装置，利用有机溶剂萃取进行乙酰胆碱酯酶抑制测定，快速检测食品中的农药残留
DOI：
10.1016/j.aca.2019.11.067
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发表时间：
2020-03-01
影响因子：
6.2
通讯作者：
Lu, Chengyin
中科院分区：
文献类型：
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作者：
Jin, Lili;Hao, Zhenxia;Lu, Chengyin
大多数有机溶剂与乙酰胆碱酯酶（AChE）抑制法的不相容性通常限制了样品前处理中农药的提取效率，这可能导致真实的世界样品评估中的假阴性。在此，已经开发了一种新的方法，用于通过有机溶剂萃取结合自发原位溶剂蒸发在微流体纸基分析装置上进行改进的AChE抑制测定。酶的预固定过程被省去，AChE被添加到采样步骤后的系统中，直到一个完整的原位溶剂蒸发过程中进行的芯片上。6种有机磷和氨基甲酸酯类农药的IC 50值表明，新方法完全消除了溶剂对AChE行为的影响。最重要的是，在食品样品测量中的分析性能得到了显着提高。采用乙腈代替水对生菜样品进行预处理，可降低基质效应。不同农药的研究表明，显着减少歧视的影响，样品预处理回收率的新方法。辛硫磷加标生菜样品的回收率为（107.5 ± 14.2）%，而水基提取样品的回收率为（18.6 ± 1.4）%。加标水和苹果汁样品西维因浓度低至0.02毫克L-1，也成功地识别与本方法通过视觉检测。这是第一个报告的直接采样的有机提取物的乙酰胆碱酯酶抑制分析芯片上，它可能会提供一个新的视角，真实的世界样品评估涉及生物试剂。(C)2019 Elsevier B. V.版权所有。
The incompatibility of most organic solvents with acetylcholinesterase (AChE) inhibition assay normally limits pesticide extraction efficiency in sample pretreatment, which might cause false negatives in real world sample assessment. Herein, a novel method has been developed for an improved AChE inhibition assay via organic solvent extraction combined spontaneous in situ solvent evaporation on microfluidic paper-based analytical devices. Enzyme pre-immobilization procedure was spared and AChE was added to the system after sampling step until a complete in-situ solvent evaporation process was performed on chip. IC50 levels of the six investigated organophosphate and carbamate pesticides indicated a completely eliminated influence of solvents on AChE behavior with the new method. Most importantly, analytical performances were significantly improved in food sample measurements. Reduction in matrix effect was observed when acetonitrile was adopted for lettuce sample pretreatment instead of water. Studies on different pesticides suggested a remarkably decreased discrimination effect on recoveries from sample pretreatment with the new developed method. The recovery level for phoxim spiked head lettuce samples reached (107.5 +/- 14.2) %, in comparison with that of (18.6 +/- 1.4) % from water-based extraction. Spiked water and apple juice samples with carbaryl concentration of as low as 0.02 mg L-1 were also successfully recognized with the present method by visual detection. This is the first report on direct sampling of organic extracts for AChE inhibition assay on-chip and it might provide a new perspective for real world sample assessments involving bio-reagents. (C) 2019 Elsevier B.V. All rights reserved.