NEUROPROTECTIVE EFFICACY OF SUBCUTANEOUS INSULIN-LIKE GROWTH FACTOR-I ADMINISTRATION IN NORMOTENSIVE AND HYPERTENSIVE RATS WITH AN ISCHEMIC STROKE
NEUROPROTECTIVE EFFICACY OF SUBCUTANEOUS INSULIN-LIKE GROWTH FACTOR-I ADMINISTRATION IN NORMOTENSIVE AND HYPERTENSIVE RATS WITH AN ISCHEMIC STROKE
复制标题
DOI：
10.1016/j.neuroscience.2013.07.016
复制
发表时间：
2013-10-10
期刊：
影响因子：
3.3
通讯作者：
Kooijman, R.
中科院分区：
文献类型：
--
作者：
de Geyter, D.;Stoop, W.;Kooijman, R.
本研究的目的是在缺血性中风大鼠模型中测试胰岛素样生长因子-I (IGF-I) 作为神经保护剂的作用，并比较其对清醒血压正常和自发性高血压大鼠的神经保护作用。使用内皮素-1 大鼠模型对缺血性中风的两种大鼠品系进行皮下注射 IGF-I 的作用进行研究。使用神经缺陷评分来测量运动感觉功能。通过甲酚紫染色评估梗塞大小。皮下注射IGF-I可显着减少血压正常大鼠的梗塞体积并增加运动感觉功能。在这些大鼠中，IGF-I 不调节纹状体中的血流，并且通过 GFAP 染色评估对星形胶质细胞的活化没有影响。在高血压大鼠中，IGF-I 的保护作用较小，而且并不总是显着。此外，IGF-I 显着降低高血压大鼠皮质中的小胶质细胞活化，但在正常血压大鼠中则不然。需要更详细的研究来查明 IGF-I 减少小胶质细胞活化是否会导致 IGF-I 治疗功效受损。事实上，我们之前已经证明，与对照大鼠相比，高血压大鼠的小胶质细胞具有不同的特性，因为它们对缺血性中风和脂多糖的反应性降低。 (c) 2013 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
The aim of this study was to test the insulin-like growth factor-I (IGF-I) as a neuroprotective agent in a rat model for ischemic stroke and to compare its neuroprotective effects in conscious normotensive and spontaneously hypertensive rats. The effects of subcutaneous IGF-I injection were investigated in both rat strains using the endothelin-1 rat model for ischemic stroke. Motor-sensory functions were measured using the Neurological Deficit Score. Infarct size was assessed by Cresyl Violet staining. Subcutaneous administration of IGF-I resulted in significantly reduced infarct volumes and an increase in motor-sensory functions in normotensive rats. In these rats, IGF-I did not modulate blood flow in the striatum and had no effect on the activation of astrocytes as assessed by GFAP staining. In hypertensive rats, the protective effects of IGF-I were smaller and not always significant. Furthermore, IGF-I significantly reduced microglial activation in the cortex of hypertensive rats, but not in normotensive rats. More detailed studies are required to find out whether the reduction by IGF-I of microglial activation contributes to an impairment IGF-I treatment efficacy. Indeed, we have shown before that microglia in hypertensive rats have different properties compared to those in control rats, as they exhibit a reduced responsiveness to ischemic stroke and lipopolysaccharide. (c) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.