Expression and relative abundance of short transient receptor potential channels in the rat renal microcirculation
Expression and relative abundance of short transient receptor potential channels in the rat renal microcirculation
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DOI：
10.1152/ajprenal.00338.2003
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发表时间：
2004-03-01
影响因子：
4.2
通讯作者：
Arendshorst, WJ
中科院分区：
文献类型：
--
作者：
Facemire, CS;Mohler, PJ;Arendshorst, WJ
在肾微循环的阻力血管中，血管收缩激素引起的血管平滑肌细胞(VSMC)内钙浓度的升高，是由钙离子内流引起的。短瞬时受体电位(TRPC)通道在哺乳动物组织中广泛表达，被认为是包括VSMCs在内的多种细胞类型中电压非依赖性阳离子进入的介体。TRPC基因家族的7个成员(TRPC1-7)编码亚基蛋白，这些亚基蛋白被认为形成同源和异源四聚体通道，根据它们的亚基组成有不同的调节。在本研究中，我们证明了在新鲜分离的大鼠肾阻力血管、肾小球和主动脉中TRPC mRNA和蛋白的相对丰富。TRPC1、3、4、5、6mRNA和蛋白在肾阻力血管和主动脉中均有表达，而TRPC2和TRPC7mRNA均不表达。TRPC1、3、5、6蛋白表达于肾小球。在肾阻力血管中，TRPC3和TRPC6的蛋白水平明显较高，约为主动脉的6到8倍。这些数据表明，TRPC3和TRPC6可能在调节肾阻力血管电压非依赖性钙离子内流中发挥作用，而肾阻力血管的功能与主动脉不同。
In the resistance vessels of the renal microcirculation, store- and/or receptor-operated calcium entry contribute to the rise in vascular smooth muscle cell (VSMC) intracellular calcium concentration in response to vasoconstrictor hormones. Short transient receptor potential ( TRPC) channels are widely expressed in mammalian tissues and are proposed mediators of voltage-independent cation entry in multiple cell types, including VSMCs. The seven members of the TRPC gene family (TRPC1- 7) encode subunit proteins that are thought to form homo- and heterotetrameric channels that are differentially regulated depending on their subunit composition. In the present study, we demonstrate the relative abundance of TRPC mRNA and protein in freshly isolated rat renal resistance vessels, glomeruli, and aorta. TRPC1, 3, 4, 5, and 6 mRNA and protein were detected in both renal resistance vessels and aorta, whereas TRPC2 and TRPC7 mRNA were not expressed. TRPC1, 3, 5, and 6 protein was present in glomeruli. TRPC3 and TRPC6 protein levels were significantly greater in the renal resistance vessels, about six- to eightfold higher than in aorta. These data suggest that TRPC3 and TRPC6 may play a role in mediating voltage-independent calcium entry in renal resistance vessels that is functionally distinct from that in aorta.