Immunologic activation during pregnancy: Serial measurement of lymphocyte phenotype and serum activation molecules in HIV-infected and uninfected women
Immunologic activation during pregnancy: Serial measurement of lymphocyte phenotype and serum activation molecules in HIV-infected and uninfected women
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DOI：
10.1016/s0165-0378(97)00018-1
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发表时间：
1997-06-01
影响因子：
3.4
通讯作者：
Plaeger, S
中科院分区：
文献类型：
--
作者：
Mikyas, Y;Aziz, N;Plaeger, S
免疫学改变发生在怀孕期间，但怀孕对HIV感染的影响是有争议的。我们在99名感染者和46名未感染者中，对妊娠期和产后6个月内可能影响HIV疾病的一些免疫学改变进行了研究。免疫表型定量的主要淋巴细胞亚群，并确定表达的活化和粘附分子的T细胞进行了使用三色染色和激光流式细胞术。血清新蝶呤，β 2-微球蛋白，和肿瘤坏死因子-α（TNF α）定量使用商业免疫测定。将HIV阳性妊娠女性与未感染妊娠受试者以及健康、HIV血清阴性非妊娠女性对照的参考范围进行比较。与非妊娠对照组相比，HIV阴性妊娠妇女的CD4和CD8 T细胞亚群均增加。在HIV感染的孕妇中，与HIV阴性孕妇和非孕妇相比，CD4 T细胞较低，CD8细胞升高。两组妇女在怀孕期间和产后亚群水平稳定。在妊娠后期发现外周免疫激活的证据。HIV阴性妇女妊娠期间CD8细胞上的HLA-DR和CD38活化抗原、血清新蝶呤和β 2-微球蛋白升高。这些免疫激活的相关性在HIV感染的孕妇中增加，并在怀孕期间进一步增加，与未感染孕妇中观察到的变化平行。这些免疫学改变可能直接或间接地增强病毒复制，影响HIV疾病的长期病程。(C)1997 Elsevier Science爱尔兰有限公司
Immunologic alterations occur during pregnancy, but the effect of pregnancy on HIV infection is controversial. We characterized some of the immunologic alterations with potential to influence HIV disease in 99 infected and 46 uninfected women during pregnancy and up to 6 months post-partum. Immunophenotyping to quantitate the major lymphocyte subsets and determine expression of activation and adhesion molecules on T cells was performed using 3-color staining and laser flow cytometry. Serum neopterin, beta 2-microglobulin, and tumor necrosis factor-alpha (TNF alpha) were quantitated using commercial immunoassays. HIV+ pregnant women were compared to uninfected pregnant subjects and to reference ranges established on healthy, HIV-seronegative non-pregnant female controls. Both CD4 and CD8 T cell subsets were increased in HIV-negative pregnant women compared to non-pregnant controls. In HIV-infected pregnant women, CD4 T cells were low and CD8 cells were elevated compared to HIV-negative pregnant and non-pregnant women. Levels of subsets were stable during pregnancy and postpartum in both groups of women. Evidence of peripheral immune activation was found during the later stages of pregnancy. Increases in HLA-DR and CD38 activation antigens on CD8 cells, serum neopterin and beta-2-microglobulin were seen during pregnancy in HIV-negative women. These correlates of immune activation were increased in HIV-infected pregnant women and increased further during pregnancy, paralleling changes seen in uninfected pregnant women. These immunologic alterations may directly or indirectly enhance viral replication, impacting the long-term course of HIV disease. (C) 1997 Elsevier Science Ireland Ltd.