Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro
Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro
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DOI：
10.1002/ijc.23148
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发表时间：
2008-02-01
影响因子：
6.4
通讯作者：
Gill, Chris I. R.
中科院分区：
文献类型：
--
作者：
Hashim, Yumi Z. H. -Y.;Rowland, Ian R.;Gill, Chris I. R.
对人类、动物和细胞系统的研究表明，初榨橄榄油中的酚类物质能够抑制癌变的几个阶段，包括转移。侵袭级联反应包括细胞附着于细胞外基质组分或基底膜，通过蛋白水解酶降解基底膜和细胞通过修饰的基质迁移。在本研究中，我们研究了从初榨橄榄油（OVP）中提取的酚类物质及其主要成分：羟基酪醇（3，4-二羟基苯基乙醇），酪醇（对羟基苯基乙醇），松醇和咖啡酸的影响。在Matrigel侵袭测定中测试这些酚类物质对HT 115人结肠癌细胞侵袭的影响。OVP及其化合物表现出不同的剂量相关的抗侵袭作用。在25 μ g/ml OVP和等效剂量的单个化合物下，在对照的45-55%的范围内观察到显著的抗侵袭作用。重要的是，OVP，而不是分离的酚类物质，显着减少总细胞数在基质胶侵袭测定。对细胞活力没有显示出显著的影响，表明基质胶侵袭测定中细胞数量的减少不是由于细胞毒性。对细胞附着于塑料基质也没有显著影响，表明细胞外基质在调节OVP的抗侵袭作用中的重要性。总之，这项研究的结果表明，初榨橄榄油中的酚类物质具有抑制结肠癌细胞侵袭的能力，其作用可能是在不同水平的侵袭级联中介导的。(c)2007 Wiley-Liss，Inc.
Studies in human, animal and cellular systems suggest that phenols from virgin olive oil are capable of inhibiting several stages in carcinogenesis, including metastasis. The invasion cascade comprises cell attachment to extracellular matrix components or basement membrane, degradation of basement membrane by proteolytic enzymes and migration of cells through the modified matrix. In the present study, we investigated the effect of phenolics extracted from virgin olive oil (OVP) and its main constituents: hydroxytyrosol (3,4-dihydroxyphenylethanol), tyrosol (p-hydroxyphenylethanol), pinoresinol and caffeic acid. The effects of these phenolics were tested on the invasion of HT115 human colon carcinoma cells in a Matrigel invasion assay. OVP and its compounds showed different dose-related anti-invasive effects. At 25 mu g/ml OVP and equivalent doses of individual compounds, significant anti-invasive effects were seen in the range of 45-55% of control. Importantly, OVP, but not the isolated phenolics, significantly reduced total cell number in the Matrigel invasion assay. There were no significant effects shown on cell viability, indicating the reduction of cell number in the Matrigel invasion assay was not due to cytotoxicity. There were also no significant effects on cell attachment to plastic substrate, indicating the importance of extracellular matrix in modulating the anti-invasive effects of OVP. In conclusion, the results from this study indicate that phenols from virgin olive oil have the ability to inhibit invasion of colon cancer cells and the effects may be mediated at different levels of the invasion cascade. (c) 2007 Wiley-Liss, Inc.