In the presence of ferritin, visible light induces lipid peroxidation of the porcine photoreceptor outer segment
In the presence of ferritin, visible light induces lipid peroxidation of the porcine photoreceptor outer segment
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DOI：
10.1080/10715760600555027
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发表时间：
2006-08-01
影响因子：
3.3
通讯作者：
Matsugo, Seiichi
中科院分区：
文献类型：
--
作者：
Ohishi, Kentaro;Zhang, Xiao Mei;Matsugo, Seiichi
我们研究了可见光和铁蛋白对猪光感受器外节(POS)部分的脂质过氧化的协同作用。含有POS组分和马脾铁蛋白的反应混合物在17,000 lx的白色荧光灯下照射或在37℃的黑暗条件下孵育。用硫代巴比妥酸法和亚铁氧化/二甲酚橙法评价脂质过氧化。辐照诱导的脂质过氧化反应受辐照剂量、材料酸度等实验因素的影响。当停止照射时，脂质过氧化也停止；此后，再次照射引起脂质过氧化。此外，铁络合剂去铁胺或羟基自由基清除剂二甲基硫脲可抑制这种脂质过氧化，这表明脂质过氧化涉及铁蛋白释放的铁离子通过Fenton反应产生的羟基自由基。在黑暗条件下或在没有铁蛋白的情况下，脂质过氧化不发生。这项研究表明，可见光诱导的POS组分在铁蛋白存在下的脂质过氧化可能参与了人类视网膜退行性疾病的病因学，因为人类视网膜终生暴露在光照下。
We studied the synergistic effect of visible light and ferritin on the lipid peroxidation on a fraction of porcine photoreceptor outer segment (POS). Reaction mixtures containing the POS fraction and horse spleen ferritin were irradiated under white fluorescent light mainly at 17,000 lx or incubated under dark conditions at 37 degrees C. The lipid peroxidation was evaluated by both the thiobarbituric acid method and the ferrous oxidation/xylenol orange method. The irradiation-induced lipid peroxidation was affected by some experimental factors such as the irradiation dose and acidity of the material. When the irradiation was stopped, the lipid peroxidation was also stopped; thereafter, the re-irradiation induced lipid peroxidation. Moreover, this lipid peroxidation was inhibited by desferrioxamine, an iron chelator, or by dimethylthiourea, a hydroxyl radical scavenger, suggesting that the lipid peroxidation involves hydroxyl radicals generated via the Fenton reaction by iron ion released from ferritin. The lipid peroxidation did not take place under dark conditions or in the absence of ferritin. This study suggested the possibility that the visible light-induced lipid peroxidation of the POS fraction in the presence of ferritin may participate in the etiology of human retinal degenerative diseases as the human retina is exposed to light for life.