meis1 regulates the development of endothelial cells in zebrafish
meis1 regulates the development of endothelial cells in zebrafish
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DOI：
10.1016/j.bbrc.2008.07.075
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发表时间：
2008-10-03
影响因子：
3.1
通讯作者：
Suzuki, Takeshi
中科院分区：
文献类型：
--
作者：
Minehata, Ken-ichi;Kawahara, Atsuo;Suzuki, Takeshi
在脊椎动物的发育过程中，内皮细胞的分化与血管的形成密切相关。这些过程需要血管内皮生长因子（vegf）介导的信号通路。本研究表明，原癌基因meis1在斑马鱼血管发育中起重要作用。反义meis1 morpholino （meis1mo）敲低meis1可导致节段间血管（ISV）形成受损。在meis1变异体中，背主动脉（DA）中动脉标记物的表达减少，DA和后主干静脉（PCV）中静脉标记物的表达增加，提示动脉发育存在缺陷。此外，vegf受体flk1的表达在这些胚胎中显著降低。有趣的是，注入f1k1 mo的胚胎表现出与meis1突变体相似的缺陷。因此，这些结果暗示meis1是一种参与内皮细胞发育的新型调节因子，可能影响vegf信号通路。(c) 2008爱思唯尔公司版权所有。
The differentiation of endothelial cells is tightly connected with the formation of blood vessels during vertebrate development. The signaling pathways mediated by vascular endothelial growth factor (vegf) are required for these processes. Here we show that a proto-oncogene, meis1, plays important roles in the vascular development in zebrafish. Knockdown of meis1 by anti-sense meis1 morpholino (meis1 MO) led to the impairment of intersegmental vessel (ISV) formation. In meis1 morphants, the expression of an artery marker was reduced in dorsal aorta (DA), and the expression of vein markers was expanded in DA and posterior cardinal vein (PCV), suggesting the defects on artery development. Furthermore, the expression of vegf receptor, flk1, was significantly decreased in these embryos. Interestingly, f1k1 MO-injected embryos exhibited similar defects as meis1 morphants. Thus, these results implicate that meis1 is a novel regulator involved in endothelial cell development, presumably affecting the vegf signaling pathway. (c) 2008 Elsevier Inc. All rights reserved.