Endoscope disinfection using chlorine dioxide in an automated washer-disinfector
Endoscope disinfection using chlorine dioxide in an automated washer-disinfector
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DOI：
10.1016/j.jhin.2006.01.020
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发表时间：
2006-07-01
影响因子：
6.9
通讯作者：
Kohno, S.
中科院分区：
文献类型：
--
作者：
Isomoto, H.;Urata, M.;Kohno, S.
虽然2%戊二醛通常是内镜消毒的一线试剂，但其不良反应在工作人员中很常见，并且对某些分枝杆菌和孢子菌的有效性较差。二氧化氯是一种可能的替代品，目前已有一种装有这种药剂的自动清洗消毒器。本研究旨在探讨二氧化氯在上消化道检查后内镜消毒中的应用效果。在存在或不存在牛血清白蛋白（BSA）的情况下，在高（600 ppm）和低（30 ppm）浓度下，对各种微生物（包括铜绿假单胞菌、幽门螺杆菌、鸟胞内分枝杆菌和枯草芽孢杆菌）的二氧化氯溶液的体外杀微生物特性进行了检查。内镜手术后立即和应用于加入30 ppm二氧化氯的自动洗消机5 min后，感染性病原体、血液、H.分别用培养法、敏感性试纸条法、聚合酶链反应（PCR）法和逆转录-PCR法检测pylori ureA基因DNA和HCV-RNA。30 ppm的二氧化氯对大多数微生物具有相同的杀微生物活性，对M.细胞内病毒和B.与2%戊二醛相比，对枯草杆菌的杀菌效果较好，但BSA的污染影响了二氧化氯的杀菌性能。应用自动洗消器/二氧化氯系统后，消除了微生物、血液和细菌DNA的内镜污染。因此，二氧化氯是戊二醛的潜在替代品。使用与二氧化氯兼容的自动再处理器，加上彻底的预清洁，可以提供有效，更快和更少的内窥镜消毒问题。(c)2006年，医院感染协会。由爱思唯尔有限公司出版。保留所有权利。
Although 2% glutaraldehyde is often the first-line agent for endoscopic disinfection, its adverse reactions are common among staff and it is less effective against certain mycobacteria and spore-bearing bacteria. Chlorine dioxide is a possible alternative and an automated washer-disinfector fitted with this agent is currently available. This study was conducted to evaluate the effectiveness of chlorine dioxide in endoscopic disinfection after upper gastrointestinal examination. In vitro microbicidal properties of chlorine dioxide solutions were examined at high (600 ppm) and low (30 ppm) concentrations against various microbes including Pseudomonas aeruginosa, Helicobacter pylori, Mycobacterium avium-intracellulare and Bacillus subtilis in the presence or absence of bovine serum albumin (BSA). Immediately following endoscopic procedures and after application to the automated reprocessor incorporating chlorine dioxide at 30 ppm for 5 min, endoscopic contamination with infectious agents, blood, H. pylori ureA gene DNA and HCV-RNA was assessed by cultivation, sensitive test tape, polymerase chain reaction (PCR) and reverse transcriptase-PCR analysis, respectively. Chlorine dioxide at 30 ppm has equivalent microbicidal activity against most microbes and faster antimicrobial effects on M. avium-intracellulare and B. subtilis compared with 2% glutaraldehyde, but contamination with BSA affected the microbicidal properties of chlorine dioxide. Endoscopic contamination with microbes, blood and bacterial DNA was eliminated after application of the automated reprocessor/chlorine dioxide system. Thus, chlorine dioxide is a potential alternative to glutaraldehyde. The use of automated reprocessors with compatibility to chlorine dioxide, coupled with thorough pre-cleaning, can offer effective, faster and less problematic endoscopic disinfection. (c) 2006 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.