THE UNUSUAL CONFORMATION ADOPTED BY THE ADENINE TRACTS IN KINETOPLAST DNA
THE UNUSUAL CONFORMATION ADOPTED BY THE ADENINE TRACTS IN KINETOPLAST DNA
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DOI：
10.1016/0092-8674(87)90702-1
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发表时间：
1987-03-27
期刊：
影响因子：
64.5
通讯作者：
TULLIUS, TD
中科院分区：
文献类型：
--
作者：
BURKHOFF, AM;TULLIUS, TD
为了确定使着丝体DNA弯曲的结构特征，我们研究了麻豆着丝体DNA中的13个腺嘌呤束。用羟基自由基切割DNA，分析了A束的结构。羟基自由基对DNA主链的反应性在5‘’内逐渐降低。3”方向的每个A束。富t链的切割模式与富a链的模式偏移了1或2 bp。质粒pBR322限制性内切片段的A束具有与着丝体A束相同的切割模式。我们解释这些实验表明，在A束中，小凹槽的宽度从5”开始平滑地减小。3英寸末端的A束。
To determine the structure features responsible for the curvature of kinetoplast DNA, we studied 13 adenine tracts in Crithidia fasciculata kinetoplast DNA. The structures of the A tracts were analyzed by cutting the DNA with hydroxyl radical. Reactivity of hydroxyl radical toward the DNA backbone progressively decreased in the 5'' .fwdarw. 3'' direction of each A tract. The cutting pattern of the T-rich strand was offset by 1 or 2 bp from the pattern on the A-rich strand. An A tract in a restriction fragment from plasmid pBR322 had the same cuting pattern as the kinetoplast A tracts. We interpret these experiments to show that in A tracts the width of the minor groove decreases smoothly from the 5'' .fwdarw. 3'' end of the A tract.