Bright photoactivatable fluorophores for single-molecule imaging
Bright photoactivatable fluorophores for single-molecule imaging
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DOI：
10.1038/nmeth.4034
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发表时间：
2016-12-01
期刊：
影响因子：
48
通讯作者：
Lavis, Luke D.
中科院分区：
文献类型：
--
作者：
Grimm, Jonathan B.;English, Brian P.;Lavis, Luke D.
小分子荧光团是先进成像实验的重要工具。我们之前报道了一种改进小的、细胞透过性荧光团的通用方法，该方法产生了含有氮杂环丁烷的‘Janelia Fluor’(JF)染料。在这里，我们通过合成与活细胞标记策略兼容的可光激活的衍生物来提炼和扩展这些染料的用途。一旦被激活，这些衍生化合物保持了JF染料的卓越亮度和光稳定性，从而改进了单粒子跟踪和简化的定位显微镜实验。
Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.