The longin domain regulates subcellular targeting of VAMP7 in Arabidopsis thaliana
The longin domain regulates subcellular targeting of VAMP7 in Arabidopsis thaliana
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DOI：
10.1016/j.febslet.2005.04.022
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发表时间：
2005-05
期刊：
影响因子：
3.5
通讯作者：
T. Uemura;Masa H. Sato;K. Takeyasu
中科院分区：
文献类型：
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作者：
T. Uemura;Masa H. Sato;K. Takeyasu
SNAREs（可溶性n -乙基马来酰亚胺敏感因子附着蛋白受体）位于特定的细胞器膜上，通过介导特定的膜融合来确保正确的囊泡运输。根据氨基酸序列的相似性和特定的保守残基，SNAREs被称为R- SNAREs或Q-SNAREs。所有的拟南芥R-SNAREs都有一个n端结构域，称为长蛋白结构域（LD）。在这项研究中，我们研究了拟南芥R-SNAREs的空泡靶向机制。液泡定位的AtVAMP711被用作gfp标记的嵌合蛋白的母蛋白，这些嵌合蛋白连接到其他细胞器定位的R-SNAREs的LD、SNARE motif （SNM）和跨膜结构域（TMD）。结果表明，虽然TMD与液泡靶向无关，但完整的LD对于液泡和亚细胞靶向至关重要。
SNAREs (soluble N-ethyl-maleimide sensitive factor attachment protein receptors) which locate on the specific organelle membrane assure the correct vesicular transport by mediating specific membrane fusions. SNAREs are referred to as R- or Q-SNAREs on the basis of the amino acid sequence similarities and specific conserved residues. All of the Arabidopsis R-SNAREs have a N-terminal domain, called the longin domain (LD). In this study, we investigated the vacuolar targeting mechanism of Arabidopsis R-SNAREs. The vacuolar localized AtVAMP711 was used as the mother protein of GFP-tagged chimeric proteins joined to several domains such as the LD, the SNARE motif (SNM) and the transmembrane domain (TMD) of other organelle-localized R-SNAREs. The results showed that, whereas the TMD is not relevant for the vacuolar targeting, a complete LD is essential for the vacuolar and subcellular targeting.