Chromatographic and Electrophoretic Techniques
Chromatographic and Electrophoretic Techniques
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色谱和电泳技术
DOI：
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发表时间：
1961
期刊：
影响因子：
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通讯作者：
Francis Martin
中科院分区：
文献类型：
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作者：
Francis Martin
第一章对血浆蛋白质的化学作了充分的说明。当然，这一节本可以扩大，但这样做会破坏这本书的平衡。对这一章的研究将很好地介绍更详细的原创文献。第二章介绍了测定蛋白质的化学方法。第三章--“检测方法”--描述了盐析、盐析、低浓度有机溶剂和电解液的各种组合沉淀等步骤。Cohn和他的同事们的研究提供了关于血浆蛋白的许多现有知识，其中包括蛋白质(在自由溶液和支持介质中)、免疫电泳和超速离心法。第四章“临床化学方法”和第五章“血浆蛋白的临床意义”讨论了依赖于血浆蛋白组成和稳定性变化的各种“经验”测试的实际细节和对结果的解释。这位评论者给人的印象是，作者所在部门对这些测试的关注比英国通常所做的要多。第六章，‘异常蛋白缺乏症和副蛋白缺乏症的临床意义’，重复了前面章节中的一些材料，但在作者看来，这是本书中最好的。
Chapter I contains an adequate account of the chemistry of the plasma proteins. This section could, of course, have been expanded, but to have done so would have upset the balance of the book. Study of this chapter would be a good introduction to the more detailed original literature. The chemical methods of determining proteins are described in Chapter II. Chapter III-' Methods of Examination '-describes the procedures such as salting-in, salting-out, precipitation by various combinations of organic solvents at low concentration and electrolytes (cf. the studies of E. J. Cohn and his colleagues), electrophoresis (in free solution and on supporting media), immunoelectrophoresis and ultracentrifugation, which have provided so much of the present body of knowledge concerning the plasma proteins. Chapter IV, 'Clinical Chemical Methods ', and Chapter V, ' Clinical Significance of the Plasma Proteins ', deal with the practical details and the interpretation of the results of the various ' empirical' tests which depend upon changes in the composition and stability of the plasma proteins. The reviewer was left with the impression that more attention is paid to these tests in the author's departments than is usually done in Great Britain. Chapter VI, 'Clinical Significance of the Dysproteinaemias and Paraproteinaemias', reduplicates some of the material given in the earlier chapters but is, in the present writer's opinion, the best in the book.