Reconstitution of functionally efficient SecA-dependent protein-conducting channels: Transformation of low-affinity SecA-liposome channels to high-affinity SecA-SecYEG-SecDF.YajC channels
Reconstitution of functionally efficient SecA-dependent protein-conducting channels: Transformation of low-affinity SecA-liposome channels to high-affinity SecA-SecYEG-SecDF.YajC channels
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DOI：
10.1016/j.bbrc.2013.01.042
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发表时间：
2013-02-15
影响因子：
3.1
通讯作者：
Tai, Phang C.
中科院分区：
文献类型：
--
作者：
Hsieh, Ying-hsin;Zhang, Hao;Tai, Phang C.
先前的工作表明，单独SecA可以促进脂质体中的蛋白质易位和离子通道活性，并且SecYEG增加效率以及信号肽特异性。我们现在报告说，SecDF.YajC进一步增加易位和离子通道的活动。重构的SecA-SecYEG-SecDF. YajC-脂质体的这些活性与天然膜的活性几乎相同，表明重构的功能性高亲和力蛋白传导通道从低亲和力SecA-通道转化。爱思唯尔公司出版
Previous work showed that SecA alone can promote protein translocation and ion-channel activity in liposomes, and that SecYEG increases efficiency as well as signal peptide specificity. We now report that SecDF.YajC further increases translocation and ion-channel activity. These activities of reconstituted SecA-SecYEG-SecDF.YajC-liposome are almost the same as those of native membranes, indicating the transformation of reconstituted functional high-affinity protein-conducting channels from the low-affinity SecA-channels. Published by Elsevier Inc.