Extracellular pH signals affect rat vascular tone by rapid transduction into intracellular pH changes.
Extracellular pH signals affect rat vascular tone by rapid transduction into intracellular pH changes.
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细胞外 pH 信号通过快速转导至细胞内 pH 变化来影响大鼠血管张力。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
Susan Wray
中科院分区：
文献类型：
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作者：
Clare Austin;Susan Wray
1.已知细胞外pH值（pHo）的变化对血管张力产生很大影响，但所涉及的机制尚不清楚。由于细胞内pH值（pHi）的变化也会影响血管张力，因此我们研究了pH值变化对pHi和张力的影响。2.大鼠肠系膜阻力血管条上装载pH敏感荧光团SNARF 1;因此，当pH值改变时，可以同时测量张力和pHi。3.每当pHo改变时，pHi也相应改变。此外，当pHo改变时，pHi的变化比报道的在其他细胞中发生的变化更快。半峰细胞内反应时间为38 +/-4 s（n = 11）。诱导的pHi变化也比研究的许多其他细胞中的衰减少。因此，每单位pHo变化的pHi变化的比率为0.73（n = 7）。4.由pHo增加产生的pHi增加伴随着血管条张力的增加。在其他实验中，通过加入弱碱性三甲胺（40 mM），在恒定的pH值下增加pH值。这也提高了条带中的张力。相反，当pHi保持在静息值而pHo改变时，血管张力没有变化。5.可以得出结论，pH值对血管张力的影响是由于诱导细胞内pH值的变化，并且血管平滑肌细胞在功能上很好地适应于组织pH值的变化，从而使组织的血流迅速改变，以满足不断变化的需求。
1. Changes in extracellular pH (pHo) are known to produce large effects on vascular tone, but the mechanisms involved are not understood. As changes in intracellular pH (pHi) can also affect vascular tone, we have investigated the effects of changing pHo upon both pHi and tone. 2. Strips of rat mesenteric resistance vessels were loaded with the pH‐sensitive fluorophore SNARF 1; thus tension and pHi could be simultaneously measured as pHo was altered. 3. Whenever pHo was altered there was a corresponding alteration of pHi. Furthermore, when pHo was changed the pHi change was more rapid than that reported to occur in other cells. The time to half‐peak intracellular response was 38 +/‐ 4 s (n = 11). The induced pHi change was also less attenuated than in many other cells studied. Thus a ratio of 0.73 was obtained for the change in pHi per unit pHo change (n = 7). 4. An increase in pHi produced by an increase in pHo was accompanied by an increase in tension in the vascular strips. In other experiments pHi was increased at constant pHo by the addition of the weak base trimethylamine (40 mM). This also elevated tension in the strips. Conversely when pHo was changed while pHi was held at resting values, no change in vascular tone occurred. 5. It is concluded that the effects of pHo on vascular tone are due to the induced change in intracellular pH, and that the vascular smooth muscle cell is functionally well adapted to respond to changes in tissue pH, thereby allowing blood flow to a tissue to be rapidly altered to meet changing needs.
碱性pH值对肌浆网Ca2+释放和Ca2+摄取的影响。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Dettbarn,C;Palade,P
通讯作者：
Palade,P
外部 pH 值对豚鼠基底动脉平滑肌细胞离子电流的影响。
影响因子：
20.1
作者：
West,GA;Leppla,DC;Simard,JM
通讯作者：
Simard,JM