Involvement of PKA, CaMKII, PKC, MAPK/ERK and PI3K in the acute antidepressant-like effect of ferulic acid in the tail suspension test
Involvement of PKA, CaMKII, PKC, MAPK/ERK and PI3K in the acute antidepressant-like effect of ferulic acid in the tail suspension test
复制标题
DOI：
10.1016/j.pbb.2012.08.020
复制
发表时间：
2012-12-01
影响因子：
3.6
通讯作者：
Tasca, Carla Ines
中科院分区：
文献类型：
--
作者：
Bertarello Zeni, Ana Lucia;Elpo Zomkowski, Andrea Dias;Tasca, Carla Ines
阿魏酸（FA，4-羟基-3-甲氧基肉桂酸）是一种天然存在于多种植物和食品中的植物化学化合物，被批准作为抗氧化剂添加剂和食品防腐剂。它在慢性轻度应激诱导的抑郁样行为中发挥有益作用，并通过激活多巴胺能系统在悬尾试验（TST）中产生急性抗抑郁样作用。本研究的目的是调查可能参与的信号通路的抗抑郁样作用的急性和口服给药的FA，在TST小鼠。口服施用FA（0.01 mg/kg，p.o.）通过用H-89（1 μ g/部位，i. c. v..，PKA抑制剂）、KN-62（1 μ g/位点，i. c. v.，CaMKII的抑制剂），GF 109203 X（5 ng/位点，i. c. v.，PKC抑制剂），U 0126（5 μ g/位点，i. c. v.，MAPK/ERK抑制剂）或LY 294002（10 nmol/位点，i. c. v.. PI 3 K的抑制剂），所有这些都涉及神经营养信号通路。结果表明，FA通过激活与神经可塑性、神经发生和细胞存活相关的信号通路，在小鼠TST中发挥抗抑郁样作用。(C)2012 Elsevier Inc. All rights reserved.
Ferulic acid (FA, 4-hydroxy-3-methoxycinnamic acid) is a phytochemical compound naturally present in several plants and foods that is approved as an antioxidant additive and food preservative. It exerts a beneficial action in chronic mild stress-induced depressive-like behavior and produces an acute antidepressant-like effect in the tail suspension test (TST) through the activation of the serotonergic system. This study was aimed at investigating the possible involvement of signaling pathways in the antidepressant-like effect of acute and oral administration of FA, in the TST in mice. The anti-immobility effect of orally administered FA (0.01 mg/kg, p.o.) was prevented by pretreatment of mice with H-89 (1 mu g/site, i.c.v.. an inhibitor of PKA), KN-62 (1 mu g/site, i.c.v., an inhibitor of CaMKII), GF109203X (5 ng/site, i.c.v., an inhibitor of PKC), U0126 (5 mu g/site, i.c.v., an inhibitor of MAPK/ERK) or LY294002 (10 nmol/site, i.c.v.. an inhibitor of PI3K), all involved with neurotrophic signaling pathways. The results demonstrated that FA exerts antidepressant-like effect in the TST in mice, through the activation of signaling pathways related to neuroplasticity, neurogenesis and cell survival. (C) 2012 Elsevier Inc. All rights reserved.