Cloning and gene expression of hexokinase I and II in the chicken skeletal muscle
Cloning and gene expression of hexokinase I and II in the chicken skeletal muscle
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DOI：
10.1111/j.1740-0929.2005.00295.x
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发表时间：
2005-10
影响因子：
2
通讯作者：
Y. Seki;Kan Sato;T. Kono;Y. Akiba
中科院分区：
文献类型：
--
作者：
Y. Seki;Kan Sato;T. Kono;Y. Akiba
为了阐明肉仔鸡骨骼肌葡萄糖稳态的独特调控机制，采用北方印迹分析方法研究了己糖激酶（HK）I和HK II基因的表达。鉴定了鸡HKI和HKII全长cDNA序列，推导的氨基酸序列与人HKI和HKII的氨基酸序列分别为87.4%和85.9%。采用逆转录-聚合酶链反应（RT-PCR）技术，在鸡骨骼肌、脑、心脏、肾脏和睾丸中检测到HKI mRNA的表达。鸡HKII mRNA在骨骼肌和心脏中高表达，而在肝、脑和肾中观察到较低的表达。禁食48小时或再喂食24小时，鸡骨骼肌HKI和HKII mRNA水平显着增加，而哺乳动物的表达不变或减少。本研究结果表明，HKI和HKII在鸡骨骼肌葡萄糖稳态调节机制中具有独特的作用，其在鸡中的作用与哺乳动物中的作用不同。
To elucidate the distinctive regulatory mechanisms of glucose homeostasis in the skeletal muscles of broiler chickens, hexokinase (HK)I and HKII gene expressions were characterized by northern blot analysis. Chicken HKI and HKII full-length cDNA sequences were identified and the deduced amino acid sequences were 87.4 and 85.9% identical to that of human HKI and HKII, respectively. Using reverse transcription-polymerase chain reaction analysis, chicken HKI mRNA was detected in the skeletal muscle, brain, heart, kidney and testis. Chicken HKII mRNA was highly expressed in the skeletal muscle and heart, whereas a lower expression was observed in the liver, brain and kidney. Fasting for 48 h or refeeding for 24 h in chickens significantly increased the skeletal muscle HKI and HKII mRNA levels in contrast to unchanged or decreased expressions in mammals. The present findings suggest that HKI and HKII have a unique role in the glucose homeostasis regulatory mechanism of chicken skeletal muscles and their role in chickens is not similar to that in mammals.