Vanadate stimulates oxidation of NADH to generate hydrogen peroxide
Vanadate stimulates oxidation of NADH to generate hydrogen peroxide
复制标题
钒酸盐刺激 NADH 氧化产生过氧化氢
DOI：
10.1016/0162-0134(84)85022-9
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发表时间：
1984
影响因子：
3.9
通讯作者：
T. Ramasarma
中科院分区：
文献类型：
--
作者：
S. Vijaya;T. Ramasarma
Vanadate in the polymeric form of decavanadate, but not other forms, stimulated oxidation of NADH to NAD+NADPH was also oxidized with comparable rates. This oxidation of NADH was accompanied by uptake of oxygen and generated hydrogen peroxide with the following stoichiometry: NADH + H++ O2→ NAD++ H2O2. The reaction followed second-order kinetics. The rate was dependent on the concentration of both NADH and vanadate and increased with decreasing pH. The reaction had an obligatory requirement for phosphate ions. Esr studies in the presence of the spin trap dimethyl pyrroline N oxide indicated the involvement of Superoxide anion as an intermediate. The reaction was sensitive to Superoxide dismutase and other scavengers of superoxide anions.
