CYCLE OF SEMINIFEROUS EPITHELIUM AND SPERMATOGENESIS IN BOVINE TESTIS
CYCLE OF SEMINIFEROUS EPITHELIUM AND SPERMATOGENESIS IN BOVINE TESTIS
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DOI：
10.1002/aja.1001400204
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发表时间：
1974-01-01
影响因子：
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通讯作者：
DESJARDINS, C
中科院分区：
文献类型：
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作者：
BERNDSTON, WE;DESJARDINS, C
本研究对发育中的精细胞顶体系统和细胞核的形态学变化进行了评价，以此作为区分牛睾丸精细胞上皮周期分期的依据。对周期性酸-希夫染色的睾丸组织进行光镜检查，可以识别精子发育（精子发生）的14个步骤。该序列的前12个步骤被用作将精原上皮的周期划分为12个不同阶段的主要标准。在此之后，通过计数生殖细胞在周期的每个阶段的数量来研究生殖细胞分化的模式。根据细胞计数，A型精原细胞主要在周期的第VII-VIII和第IX-X阶段分裂。一些A型细胞在第XII期结束时再次分裂，产生中间精原细胞，而另一些细胞则明显保持“休眠”状态，直到下一个周期。在IV期末期，中间精原细胞分裂产生b1型精原细胞，b1型精原细胞在V期末期分裂产生b2型精原细胞。初生精母细胞在第VIII期出现，在下一个周期的第XI期后期分裂形成次生精母细胞。这些细胞在第十二期末分裂形成幼精细胞。结果表明，精子发育过程中顶体系统和核形态的变化为区分精子上皮周期和研究牛睾丸精子发生过程中生殖细胞的发育模式提供了有用的标准。
Morphological changes in the acrosomic system and nuclei of developing spermatids were evaluated as a basis for classifying the stages of the cycle of the seminiferous epithelium in the bovine testis. Light microscopic examination of periodic acid‐Schiff‐stained testicular tissue permitted identification of 14 steps of spermatid development (spermiogenesis). The first 12 steps in this sequence were utilized as the major criterion to divide the cycle of the seminiferous epithelium into 12 distinct stages. Following this, the pattern of germ cell differentiation was investigated by counting the number of germ cells at each stage of the cycle. Based on cell counts, type A spermatogonia divided primarily during stages VII–VIII and IX–X of the cycle. Some type A cells divided again at the end of stage XII to produce intermediate spermatogonia, while others apparently remained “dormant” until the following cycle. At the end of stage IV, intermediate spermatogonia divided to produce type B1spermatogonia which in turn divided at the end of stage V to produce type B2spermatogonia. Primary spermatocytes appeared during stage VIII and divided late in stage XI of the following cycle to form secondary spermatocytes. These divided to form young spermatids at the end of stage XII. It was concluded that changes in the acrosomic system and nuclear morphology of developing spermatids provide useful criteria for dividing the cycle of the seminiferous epithelium into stages as well as investigating the pattern of germ cell development during spermatogenesis in the bovine testis.