Human chromosome 12 encodes a species-specific factor which increases human immunodeficiency virus type 1 tat-mediated trans activation in rodent cells.
Human chromosome 12 encodes a species-specific factor which increases human immunodeficiency virus type 1 tat-mediated trans activation in rodent cells.
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人类 12 号染色体编码一种物种特异性因子，可增加啮齿动物细胞中人类免疫缺陷病毒 1 型 tat 介导的反式激活。
DOI：
10.1128/jvi.64.9.4565-4567.1990
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发表时间：
1990
影响因子：
5.4
通讯作者：
Shank,PR
中科院分区：
文献类型：
--
作者：
Newstein,M;Stanbridge,EJ;Casey,G;Shank,PR
The human immunodeficiency virus type 1 (HIV-1) tat protein functions at a much lower level in rodent cells than in human cells. This species-specific difference in trans activation appears to be due to the lack of a functional homolog of a human cofactor for tat in rodent cells. Using HIV-1 long terminal repeat-driven human growth hormone as a reporter plasmid, we found that the tat-mediated trans activation functions at a level 5- to 20-fold lower in rodent cells than in human cells. Stable rodent-human hybrid cells containing only human chromosome 12 support a dramatically higher degree of trans activation. Thus, human chromosome 12 encodes a species-specific HIV-1 tat cofactor which, at least partially, restores high levels of tat-mediated trans activation. Chromosome 6 also appears to provide an additional factor which enhances HIV-1 tat-mediated trans activation in murine cells.
