Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis

Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis
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DOI:
10.1128/jcm.38.2.855-857.2000
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发表时间:
2000-02-01
影响因子:
9.4
通讯作者:
Taha, MK
Taha, MK
中科院分区:
医学2区
文献类型:
--
作者:
Taha, MK

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采用非培养PCR方法对225份临床标本进行脑膜炎奈瑟菌检测。PCR能正确识别和预测N.在35%的疑似样本中证实了这一诊断。当培养未能分离出N.脑膜炎
A nonculture PCR-based method to characterize Neisseria meningitidis was used to test 225 clinical specimens. PCR correctly identified and predicted the serogroups of N. meningitidis of culture-proven meningococcal diseases and confirmed this diagnosis in 35% of suspected samples. This approach could be useful when culture fails to isolate N. meningitidis.