Strontium (Sr) and silver (Ag) loaded nanotubular structures with combined osteoinductive and antimicrobial activities
Strontium (Sr) and silver (Ag) loaded nanotubular structures with combined osteoinductive and antimicrobial activities
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DOI：
10.1016/j.actbio.2015.11.046
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发表时间：
2016-02-01
影响因子：
9.7
通讯作者：
Li, Feng
中科院分区：
文献类型：
--
作者：
Cheng, Hao;Xiong, Wei;Li, Feng
两个常见的问题与骨/牙科植入物的钛表面相关：缺乏天然组织整合和相关感染。这些问题促使了大量关于这些表面改性的研究。本研究描述了一种水热处理方法，用于在钛表面制备具有不同管径的锶（Sr）和银（Ag）负载纳米管结构。通过钛纳米管（NT）的内径尺寸（分别在10 V或40 V下形成的30 nm或80 nm）来调节Sr（OH）（2）溶液中的Sr负载。通过将样品浸入1.5或2.0 M AgNO 3溶液中来调节Ag的量。Sr和Ag从NT-Ag.Sr样品中以可控和长时间的物质释放，细胞毒性可忽略不计。由于Ag的释放，观察到显著的抗菌活性。Sr掺入增强了前成骨细胞MC 3 T3-E1细胞的初始细胞粘附、迁移和增殖。锶释放还上调成骨基因的表达和诱导矿化，如在NT-Ag.Sr表面上培养的细胞中存在更多矿化的钙结节所示。在体内实验表明，锶加载的样品加速骨质疏松症和骨缺损模型中的新骨形成，证实了植入NT-Ag.Sr样品的大鼠的X射线，Micro-CT评价和组织形态计量学分析。NT-Ag.Sr样品的抗菌活性和突出的成骨特性突出了其在临床应用中的优异潜力。显著性声明广泛用于骨科和牙科领域的Ti表面存在两个常见问题，我们描述了一种制造锶（Sr）和银（Ag）的新方法，在钛表面上负载纳米管结构。这项工作的一个相关方面是长期和可控的银释放，导致对耐甲氧西林金黄色葡萄球菌（MRSA）和革兰氏阴性菌，如Escherichiacoli. Sr的延长释放加速骨缺损的填充，通过改善受损皮质骨的修复和增加骨小梁微结构的演示，优良的抗菌和抗粘附性能。我们的研究结果突出了Sr和Ag负载的纳米管结构用于临床应用的潜力。(C)2015 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Two frequent problems are associated with the titanium surfaces of bone/dental implants: lack of native tissue integration and associated infection. These problems have prompted a significant body of research regarding the modification of these surfaces. The present study describes a hydrothermal treatment for the fabrication of strontium (Sr) and silver (Ag) loaded nanotubular structures with different tube diameters on titanium surfaces. The Sr loading from a Sr(OH)(2) solution was regulated by the size of the inner diameter of the titanium nanotubes (NT) (30 nm or 80 nm, formed at 10 V or 40 V, respectively). The quantity of Ag was adjusted by immersing the samples in 1.5 or 2.0 M AgNO3 solutions. Sr and Ag were released in a controllable and prolonged matter from the NT-Ag.Sr samples, with negligible cytotoxicity. Prominent antibacterial activity was observed due to the release of Ag. Sr incorporation enhanced the initial cell adhesion, migration, and proliferation of preosteoblast MC3T3-E1 cells. Sr release also up-regulated the expression of osteogenic genes and induced mineralization, as suggested by the presence of more mineralized calcium nodules in cells cultured on NT-Ag.Sr surfaces. In vivo experiments showed that the Sr-loaded samples accelerated the formation of new bone in both osteoporosis and bone defect models, as confirmed by X-ray, Micro-CT evaluation, and histomorphometric analysis of rats implanted with NT-Ag.Sr samples. The antibacterial activity and outstanding osteogenic properties of NT-Ag.Sr samples highlight their excellent potential for use in clinical applications.Statement of SignificanceTwo frequent problems associated with Ti surfaces, widely used in orthopedic and dental arenas, are their lack of native tissue integration and risk of infection.We describe a novel approach for the fabrication of strontium (Sr) and silver (Ag) loaded nanotubular structures on titanium surfaces. A relevant aspect of this work is the demonstration of long-lasting and controllable Ag release, leading to excellent antibacterial and anti-adherent properties against methicillin-resistant Staphylococcus aureus (MRSA), and Gram-negative bacteria such as Escherichia coli.The extended release of Sr accelerates the filling of bone defects by improving the repair of damaged cortical bone and increasing trabecular bone microarchitecture. Our results highlight the potential of Sr and Ag loaded nanotubular structures for use in clinical applications. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.